检验医学与临床2019，Vol.16Issue(3)：303-305,3.DOI:10.3969/j.issn.1672-9455.2019.03.005
多重耐药鲍曼不动杆菌膜孔蛋白OprD与标准敏感株同源建模的比较
Comparison of homologous modeling between Acinetobacter baumannii membrane porin OprD and standard drug-sensitive strain
摘要
Abstract
Objective To understand the model of multidrug-resistant Acinetobacter baumannii (MDR-AB) membrane porin OprD and to compare it with standard drug-sensitive strain (SDF) .Methods Twenty-nine strains of MDR-AB isolated from the clinical sample were selected from November 2017 to May 2018.The Kirby Bauer diffusion method was used to detect its susceptibilities to antimicrobial agents;the nested-PCR was used to detect OprD, which was linked with DANMAN and performed the contrastive analysis by Blast.Results In the comparison between OprD in 29 strains of MDR-AB with SDF, the same mutation occurred, threonine (T) at 98 site was mutated into alanine (A) , and aspartic acid at 278 site was mutated into glycine (G) , which were submitted to swiss-model for constructing the model, theβ-barrel structure mutation was obviously found.Conclusion The mutation occurs in membrane porin OprD of MDR-AB, moreover this mutation may be one of factors causing multidrug resistance of AB.关键词
鲍曼不动杆菌/多重耐药/膜孔蛋白/OprDKey words
Acinetobacter baumannii/multidrug-resistance/membrane porin/OprD分类
医药卫生引用本文复制引用
杨慧健,孙杰,何雁鸿,吴腊梅..多重耐药鲍曼不动杆菌膜孔蛋白OprD与标准敏感株同源建模的比较[J].检验医学与临床,2019,16(3):303-305,3.基金项目
上海市卫生和计划生育委员会青年基金(20184Y0087) （20184Y0087）
上海市嘉定区卫计委青年基金(2017-QN-05) （2017-QN-05）
上海健康医学院附属嘉定区中心医院中青年课题(ZQN201804). （ZQN201804）
