检验医学与临床2019，Vol.16Issue(3)：316-319,4.DOI:10.3969/j.issn.1672-9455.2019.03.009
p16/Ki67双染检测在分流宫颈低度/高度鳞状上皮内病变及预测低度鳞状上皮内病变转归中的临床研究
Clinical study of p16/Ki67 double staining test in shunting cervical low/high squamous intraepithelial lesion and predicting outcome of low grade squamous intraepithelial lesion
摘要
Abstract
Objective To investigate the clinical significance of p16/Ki67 double staining in shunting low/high squamous intraepithelial lesion (LSIL/HSIL) and predicting LSIL outcome.Methods The four groups of cervicitis, LSIL, HSIL and cervical cancer specimens (102, 75, 68, 59 cases respectively) were collected from the Lianjiang Municipal People′s Hospital and Zhanjiang Municipal Central People′s Hospital as the study objects.The p16/Ki67 double staining detection results of preclinical cytology specimens were retrospectively observed.The relationship between p16/Ki67 double staining and the outcome of LSIL was observed.The efficiency of p16/Ki67 double staining for diagnosing HSIL and more (HSIL+, including HSIL and cervical cancer) was statistically analyzed.Seventy-five cases of definitely diagnosed LSIL conducted the follow up 2 years after diagnosis.The relationship between p16/Ki67 double staining detection results with LSIL outcome was analyzed.Results (1) The positive rates of p16/Ki67 double staining in NILM, ASCUS, LSIL and ASU-H+groups (including ASU-H, high grade squamous intraepithelial neoplasia, squamous cell carcinoma) were8.79％, 41.67％, 58.57％ and 91.57％ respectively.The overall difference was statistically significant (P<0.05) .There was no significant difference in the positive rate of p16/Ki67 between ASCUS group and LSIL group (P>0.008) , but there was statistically significant difference in the positive rate of p16/Ki67 between the other two groups (P<0.008) . (2) The positive rates of p16/Ki67 double staining in cervicitis, LSIL, HSIL and cervical cancer groups were 7.84％, 30.67％, 88.24％and 94.92％respectively.The overall difference was statistically significant (P<0.05) .There was no significant difference in the positive rate of p16/Ki67 between HSIL group and cervical cancer group (P>0.008) , but there was significant difference in the positive rate of p16/Ki67 between the other two groups (P<0.008) . (3) The sensitivity, specificity, coincidence rate, positive predictive value and negative predictive value of p16/Ki67 for diagnosing HSIL+ lesion were91.34％, 82.49％, 86.18％, 78.91％ and 92.99％ respectively. (4) The lesion progression rate of LSIL patients with p16/Ki67 double staining positive was 5.28 times higher than that of LSIL patients with p16/Ki67 double staining negative, the difference between them was statistically significant (P<0.05) .Conclusion The positive rate of p16/Ki67 double staining is constantly increased with the aggravation of cervical disease diagnostic results.The detection of p16/Ki67 has a certain significance in diagnosing HSIL+ lesions, shunting LSIL/HSIL and predicting the outcome of LSIL, which is worthy of being clinically promoted and applied.关键词
p16/Ki67抗原/高度鳞状上皮内病变/低度鳞状上皮内病变/宫颈癌Key words
p16/Ki67antigen/high-grade squamous intraepithelial lesion/low-grade squamous intraepithelial lesion/cervical cancer分类
医药卫生引用本文复制引用
黎法文,邱文山,崔彬,杨岚,庄妙,吕观东..p16/Ki67双染检测在分流宫颈低度/高度鳞状上皮内病变及预测低度鳞状上皮内病变转归中的临床研究[J].检验医学与临床,2019,16(3):316-319,4.基金项目
广东省湛江市科技公关计划立项课题(2017B01081). （2017B01081）
