摘要
Abstract
Objective To provide the accurately diagnostic basis for the epidemic situation of measles, rubella, etc.and to provide a basis for further formulating the strategy of measles and rubella prevention and treatment in Hechuan District.Methods The enzyme linked immunosorbent assay (ELISA) was used to detect IgM antibody in serum samples of suspected cases of measles and rubella.The real time fluorescent quantitative PCR was used to detect viral RNA nucleic acid in throat swabs of measles and rubella suspected cases.Results The IgM antibodies were detected in serum samples from 233 cases of suspected measles and rubella during 2014-2017, in which 42 cases (18.0％) were positive for measles IgM, 47 cases (20.2％) were RNA nucleic acid positive, 27 cases (11.6％) were rubella IgM antibody positive, and 58 cases (24.9％) were RNA nucleic acid positive.The onset characteristics:measles had high onset from February to July (59.5％) , the age group of less than 3 years old was easy to be infected, the positive rate was 54.8％;rubella had high onset from April to July (40.7％) and December (29.6％) , the age group of 9 months old to 3 years old were easy to be infected, and the positive rate was 14.8％.Conclusion The positive rate of the real-time PCR method is higher than that of ELISA method.In conducting the measles and rubella detection, the nucleic acid detection of throat swabs sample in early onset can improve the monitoring promptness.Strengthening the management of planned immunization, carrying out the leak detection, supplementary immunization and adult enhanced immunization, and make monitoring the epidemic situation well reach the goal for controlling and eliminating measles prevalence in Hechuan District.关键词
麻疹/风疹/IgM抗体/RNA核酸Key words
measles/rubella/IgM antibody/RNA nucleic acid分类
医药卫生
