检验医学与临床2019，Vol.16Issue(4)：433-437,5.DOI:10.3969/j.issn.1672-9455.2019.04.001
基于液质联用技术的慢性肾脏病不同阶段患者血浆代谢组学研究
Study on plasma metabonomics of patients with different stages of chronic kidney disease based on HPLC-Q/TOF technology
摘要
Abstract
Objective To analyze the plasma differential components in the patients with different stages of chronic kidney disease (CKD), and to probe the pathogenesis of CKD in order to provide a basis for previous diagnosis.Methods The metabonomic method based on HPLC-Q/TOF technology was used to draw the plasma metabolic profiles in the patients with CKD stageⅠ-Ⅲ, ⅣandⅤand healthy controls.The data mode recognition of single factor analysis of variance, multiple testing and principal component were performed by using the Agilent MPP 12.1software.The plasma differential compounds in each group were sought.The structures of different compounds were identified and the metabolic pathways were analyzed.Results The multivariate statistical analysis results showed that the plasma samples in the patients with different stages of CKD had obvious difference, and 27different compounds in the plasma such as sphingosine, taurine, protoporphyrinⅨwere found.Conclusion The metabolic pathways of bilirubin circulation, sheath lipid metabolism and steroid metabolism in plasma of the patients with different stages of CKD appear the disorder, this study provides reference for the clinical diagnosis and treatment of CKD.关键词
慢性肾脏病/代谢组学/血浆差异化合物Key words
chronic kidney disease/metabonomics/plasma different compounds分类
医药卫生引用本文复制引用
关欣,王帅,李天娇,包永睿,孟宪生..基于液质联用技术的慢性肾脏病不同阶段患者血浆代谢组学研究[J].检验医学与临床,2019,16(4):433-437,5.基金项目
辽宁省特聘教授项目[辽教发(2014187号)] （2014187号）
辽宁省高等学校优秀人才支持计划资助项目(LR2013044) （LR2013044）
