林产化学与工业2019，Vol.39Issue(1)：41-45,5.DOI:10.3969/j.issn.0253-2417.2019.01.006
银杏叶聚戊烯醇的分离纯化及其亲水衍生物的合成
Separation, Purification and Hydrophilic Derivative Synthesis of Ginkgo biloba Leaves Polyprenol
摘要
Abstract
The analytical methodology and method performance for the determination of polyprenol in Ginkgo biloba leaves were investigated for the first time. The results showed that of the high performance liquid chromatography with C18 as chromatographic column, isopropanol methanol (volume ratio, 32∶18) as mobile phase, 210 nm as detection wavelength showed high precision (RSD=0.89％) and good stability (RSD=3.26％) . In addition, the average standard recovery and RSD value of polyprenol were 97.4％ and 1.75％, respectively. Then, polyprenol with the purity of 98.6％ was obtained by extraction with n-hexane, saponification, de-waxing with ethanol and acetone, and silica gel column chromatography (V (diethyl ether)∶V (n-hexane) =3∶97) . On this basis, the terminal hydroxyl group of high-purity polyprenol was modified to synthesize aminopolyprenol. Firstly, by mitsunobu reaction with phthalimide, followed by reduction reaction with hydrazine hydrate, to obtain aminopolyprenol. The structure of amino polyprenol was characterized by infrared and nuclear magnetic resonance spectroscopy, which confirmed the aminopolyprenol was successfully synthesized.关键词
聚戊烯醇/分离/纯化/亲水改性Key words
polyprenol/separation/purification/hydrophilic modification分类
化学化工引用本文复制引用
张昌伟,马余璐,陶冉,叶建中,王成章..银杏叶聚戊烯醇的分离纯化及其亲水衍生物的合成[J].林产化学与工业,2019,39(1):41-45,5.基金项目
江苏省生物质能源与材料重点实验室项目(JSBEM-S-201708) （JSBEM-S-201708）
江苏省青年基金(SBK2018040639) （SBK2018040639）
