林产化学与工业2019，Vol.39Issue(1)：88-94,7.DOI:10.3969/j.issn.0253-2417.2019.01.013
蒸汽爆破预处理对杜仲皮活性成分和杜仲胶提取的影响
Effect of Steam Explosion on Extraction of Bioactive Components and Gutta-percha from Barks of Eucommia ulmoides Oliver
摘要
Abstract
Eucommia ulmoides Oliver bark was pretreated by steam explosion, and then the pretreated bark was hydrolyzed by cellulase to extract bioactive components and gutta-percha. Effect of steam explosion pretreatment on the bioactive components, yield and molecular weight of gutta-percha from bark of E. ulmoides Oliver were investigated. The results showed that the optimal conditions for the extraction of bioactive components from untreated barks using ethonal sonication-assisted method were 40％ ethanol, ultrasonic power 200 W, extraction temperature 40 ℃ and extraction time 40 min. When the conditions of steam explosion pretreatment were 3.25 MPa and 1 min, the yields of flavonoids and chlorogenic acid using ethonal sonication-assisted method were 26.83 and 1.22 mg/g, respectively, and the yields of flavonoids and chlorogenic acid extracted by cellulase hydrolysis were 19.17 and 0.93 mg/g, respectively. The solid residue of pretreated barks after enzymatic hydrolysis was used to extract gutta-percha. When the conditions of steam explosion pretreatment were 0.52 MPa and 15 min, the yields of gutta-percha was 5.92％, and the Mw and Mn of gutta-percha were 28.39×104 and 6.45×104, respectively.关键词
杜仲皮/蒸汽爆破/活性成分/杜仲胶/酶水解Key words
Eucommia ulmoides Oliver bark/steam explosion/bioactive components/gutta-percha/enzymatic hydrolysis分类
化学化工引用本文复制引用
魏锦锦,辛东林,陈翔,全熙宇,张军华..蒸汽爆破预处理对杜仲皮活性成分和杜仲胶提取的影响[J].林产化学与工业,2019,39(1):88-94,7.基金项目
国家林业局948项目(2015-4-59) （2015-4-59）
