临床神经外科杂志2019，Vol.16Issue(1)：11-16,6.DOI:10.3969/j.issn.1672-7770.2019.01.003
CRNDE在脑胶质瘤的差异表达及与临床、病理特征的相关性
Changes in expression levels of long-chain non-coding RNA CRNDE in glioma and its association with clinical and pathological characteristics
摘要
Abstract
Objective To investigate the expression of CRNDE in glioma of different pathological grades and adjacent non-tumor tissues, and to explore the association between CRNDE expression levels and clinical, pathological characteristics. Methods A total of 164 tumor samples and adjacent non-tumor tissues were collected from patients who underwent surgical resection in the Department of Neurosurgery, Hebei province Children''s Hospital from 2007 to 2017. Quantitative real-time PCR (qRT-PCR) were performed to detect CRNDE expression levels in adjacent non-tumor tissues and glioma tissues of different grades for comparison. Chi-square test were used to investigate the relationship between clinicopathological characteristics and CRNDE mRNA expression levels in164 glioma tissues. Univariate analysis were performed by the Kaplan-Meier method and survival curves were constructed. The effects of CRNDE expression on survival time were investigated and analyzed using Log-rank analysis. Results Compared with adjacent non-tumor tissues (1. 00 ±0. 06) , expression level of CRNDE in glioma tissues (3. 49 ± 1. 71) was highly up-regulated, and the differences were statistically significant (P ＜ 0. 05). CRNDE expression level in high-grade gliomas (Ⅲ—Ⅳ) (4. 26 ± 1. 29) was significantly higher than that in low-grade gliomas (Ⅰ—Ⅱ) (1. 51 ± 0. 86) , showing significant difference (P ＜ 0. 05). There was no correlation between CRNDE expression level and gender, age, preoperative symptoms as well as tumor necrosis, CRNDE mRNA expression level was correlated with tumor size, pathological grades of tumor and tumor recurrence. The contingency coefficients between CRNDE mRNA expression level and tumor size, tumor grade as well as tumor recurrence were 0. 2, 0. 38 and 0. 2 respectively. The 1 year survival rate in high and low CRNDE expression group were 91. 4％ and 96. 1％ (P ＜ 0. 05). The 5 year survival rate in high and low CRNDE expression group were 23. 2％ and 59. 1％ (P ＜ 0. 05).Conclusions Expression levels of CRNDE mRNA were significantly higher in human glioma tissues than in adjacent non-tumor tissues, which were associated with pathological grades of glioma. CRNDE expression levels in high-grade gliomas (Ⅲ-Ⅳ) were higher than those in low-grade gliomas (Ⅰ-Ⅱ) , suggesting significant difference. CRNDE expression level was positively correlated with WHO grading, tumor size, and tumor recurrence, other than gender and age. Overall survival time was shorter in those with high expression of CRNDE.关键词
CRNDE/脑胶质瘤/临床特征/病理特征Key words
CRNDE/glioma/clinical factors/pathological factors分类
医药卫生引用本文复制引用
景世元,杨志国,程征海,方江顺,李鑫..CRNDE在脑胶质瘤的差异表达及与临床、病理特征的相关性[J].临床神经外科杂志,2019,16(1):11-16,6.基金项目
河北省医学科学研究重点课题计划 (20150099) （20150099）
