临床神经外科杂志2019，Vol.16Issue(1)：23-28,6.DOI:10.3969/j.issn.1672-7770.2019.01.005
白藜芦醇增强5-氨基酮戊酸在人脑胶质瘤细胞中的光动力治疗作用
Enhancement of resveratrol to 5-aminolevulinic acid in human glioma cells by photodynamic therapy
摘要
Abstract
Objective To study the effect of resveratrol not only on the direct treatment of human glioma cells, but also on the excretion of protoporphyrin Ⅸ (Pp Ⅸ) from living cells by inhibiting BCRP/ABCG2 as an ABCG2 inhibitor and to evaluate the effect of resveratrol on 5-aminolevulinic acid (5-ALA) treated human glioma cells. The effect of photodynamic therapy (PDT) was evaluated.Methods Four malignant glioma cell lines (U87 MG, U251, A172 and T98G) interacted with 5-ALA (1 mM) before mix with resveratrol at different concentrations (0. 01-1. 0 μM). To study the effect of resveratrol on intracellular PpⅨ, the expression of BCRP/ABCG2 mRNA and ABCG2 protein, and the PDT in vitro. Results Resveratrol at 0. 1 μM or higher concentration could increase the level of PpⅨ in malignant brain tumor cells, which was dose-dependent in all four cell lines studied.Resveratrol not only decreased the expression of BCRP/ABCG2 mRNA, but also decreased the expression of BCRP/ABCG2 protein on cell membrane, and effectively improved the effect of photodynamic therapy for malignant brain tumors. Conclusion Resveratrol can not only inhibit the outflow of PpⅨ in malignant brain tumor cells mediated by BCRP/ABCG2, but also increase the content of PpⅨ in cells, so as to improve the efficacy of PDT.关键词
5-氨基酮戊酸/白藜芦醇/原卟啉Ⅸ/光动力治疗Key words
5-aminolevulinic acid (5-ALA)/resveratrol (Res)/protoporphyrin Ⅸ (PpⅨ)/photodynamic therapy (PDT)分类
医药卫生引用本文复制引用
孙伟,刘卫东,龚良,刘向阳,郑佳佳,钱忠心..白藜芦醇增强5-氨基酮戊酸在人脑胶质瘤细胞中的光动力治疗作用[J].临床神经外科杂志,2019,16(1):23-28,6.基金项目
上海市浦东新区科委项目 (PKJ2015-Y25) （PKJ2015-Y25）
上海市医学重点专科建设 (ZK2015A12) （ZK2015A12）
上海市浦东新区卫生系统临床高原学科建设项目 (PWYgy2018-04) （PWYgy2018-04）
