临床误诊误治2019，Vol.32Issue(3)：55-58,4.DOI:10.3969/j.issn.1002-3429.2019.03.013
miR-421下调Bcl-XL诱导宫颈癌Hela细胞凋亡的作用机制
Mechanism of miR-421 Down-regulating Bcl-XL to Induce Apoptosis in Cervical Cancer Hela Cells
摘要
Abstract
Objective To investigate the expression of miR-421 in cervical cancer and its mechanism of action on apoptosis. Methods The expression of miR-421 in cervical cancer tissues and adjacent normal cervical tissues was detected by Real-time Quantitative PCR (RT-qPCR). Cervical cancer Hela cells were selected, and miR-421 inhibitor, blank control and miR-421 inhibitor+Bcl-XL were transfected into Hela cells.The flow cytometry was used to detect the effect of miR-421 on the apoptosis of Hela cells.Dual luciferase reporter assay was used to validate the targeted regulatory relationship between miR-421 and B cell lymphoma-XL (Bcl-XL). Western Blot was used to detect the effect of miR-421 on the protein expression of Bcl-XL. Results RT-qPCR showed that the expression of miR-421 in cervical cancer tissues and adjacent normal cervical tissues was 3.13±0.26 and 1.23±0.11, respectively, and the difference was statistically significant (P＜0.05). The results of flow cytometry showed that the apoptosis rates of miR-421 inhibitor group and control group were (2.63±0.13) ％ and (12.31±0.29) ％, respectively, and the difference was statistically significant (P＜0.05). The results of dual luciferase reporter assay and Western Blot showed that the luciferase activity of wild-type Bcl-XL was significantly inhibited after inhibition of miR-421 expression, while that of mutant Bcl-XL was not significantly inhibited. After inhibiting the expression of miR-421, the protein expression level of Bcl-XL was also significantly reduced. Conclusion miR-421 induces apoptosis of cervical cancer Hela cells by down-regulating Bcl-XL.关键词
宫颈肿瘤/细胞凋亡/微RNAsKey words
Uterine cervical neoplasms/Apoptosis/MicroRNAs分类
医药卫生引用本文复制引用
王彩梅,李海香..miR-421下调Bcl-XL诱导宫颈癌Hela细胞凋亡的作用机制[J].临床误诊误治,2019,32(3):55-58,4.基金项目
陕西省自然科学基金(ZRY201609026) （ZRY201609026）
