临床与实验病理学杂志2019，Vol.35Issue(1)：14-18,5.DOI:10.13315/j.cnki.cjcep.2019.01.004
鸡尾酒抗体D2-40/CD34-CK对淋巴结检出数量不足结直肠癌标本的临床病理评价
Application of D2-40/CD34-CK cocktail antibodies for colorectal cancer with insufficient lymph node harvest
摘要
Abstract
Purpose To investigate the value of application of D2-40/CD34-CK cocktail antibodies by double immunohistochemical staining for assessment of lymphovascular invasion (LVI) and to determine its prognostic significance in colorectal cancer with insufficient lymph node harvest. Methods Specimens from 133 cases of colorectal cancer with less than 12 lymph nodes were selected. HE staining and double immunohistochemical staining of the cocktail antibodies were performed to compare the difference of the two methods in screening for LVI. The The relationship between LVI confirmed by cocktail antibody immunohistochemical staining and clinicopathological characteristics and overall survival (OS) of patients was analyzed. Results (1) The detection rates of cocktail antibody double immunohistochemical staining and HE staining for LVI were 42.9％ (57/133) and 21.8％ (29/133) with statistically significant difference (P < 0.001). (2) The presence of LVI confirmed by double staining was significantly associated with Dukes staging, depth of invasion, clinical stages, lymph node metastasis and tumor budding (P < 0.05). (3) The presence of LVI, the location and extent of LVI, and the number of tumor cells in thrombus ≥5.5 for cases with LVI ≤2 clusters, were significantly associated with OS (P < 0.05). Conclusion D2-40/CD34-CK cocktail antibodies double staining is superior to routine HE staining in assessing LVI. LVI is intimately associated with tumor stage, lymph nodes metastasis and tumor budding, and it is an independent prognostic factor for CRC patients. It should be a supplementary examination for these patients with insufficient lymph node harvest.关键词
结直肠肿瘤/鸡尾酒抗体D2-40/CD34-CK/脉管侵犯/预后/临床病理Key words
colorectal neoplasm/D2-40/CD34-CK cocktail antibodies/lymphovascular invasion/prognosis/clinicopathological分类
医药卫生引用本文复制引用
刘仙花,叶显宗,余英豪,齐兴峰,吴在增,胡顺奇,熊喜生,向娟,郑智勇,曲利娟..鸡尾酒抗体D2-40/CD34-CK对淋巴结检出数量不足结直肠癌标本的临床病理评价[J].临床与实验病理学杂志,2019,35(1):14-18,5.基金项目
国家自然科学基金青年科学基金(81301787) （81301787）
