临床与实验病理学杂志2019，Vol.35Issue(1)：27-32,6.DOI:10.13315/j.cnki.cjcep.2019.01.007
结直肠腺癌中Fascin-1、β-catenin蛋白表达及其与K-ras基因突变状态的相关性
Expression of Fascin-1 and β-catenin protein in colorectal adenocarcinoma and its relevance with K-ras gene mutations
摘要
Abstract
Purpose To examine the expression of Fascin-1 and β-catenin protein and K-ras gene mutation in colorectal adenocarcinoma, and to explore their role in progression of colorectal neoplasm and their relevance. Methods Fascin-1 and β-catenin were analyzed by use of immunohistochemistry En Vision two-step. K-ras gene mutation was detected by ARMS method.Relationship between overexpression of Fascin-1, the nuclear expression of β-catenin, and the mutations of K-ras gene and clinicopathologic parameters was analyzed, the correlation between them was also analyzed. Results In 112 colorectal adenocarcinoma samples, the overexpression rate of Fascin-1 protein was 27.7％ (31/112), significantly higher than non-neoplastic mucosa (P < 0.01). The high nuclear expression rate of β-catenin was 29.5％ (33/112) in adenocarcinoma and non-neoplastic mucosa respectively with a significant difference between two groups (P < 0.01). High expression rate of Fascin-1 protein and β-catenin were correlated significantly with lymph node metastasis (P = 0.022, P = 0.027), and TNM staging (P =0.042, P = 0.019) in colorectal adenocarcinoma. The overexpression of Fascin-1 protein was correlated with tumor location (P = 0.004). The mutation rate of K-ras gene was 34.8％ (39/112), which showed no correlation with age, gender, tumor size, grade of differentiation, lymph node metastasis and TNM staging (P> 0.05). There was a correlation between the overexpressison of Fascin-1 protein, the nuclear expression of β-catenin and the mutation of K-ras gene (rs= 0.252, rs= 0.258, P < 0.05). The overexpression of Fascin-1 protein positively correlated with the nuclear expression of β-catenin (rs= 0.213, P < 0.05). Conclusion Fascin-1 protein and β-catenin protein are involved in invasion and metastasis of colorectal cancer and are associated with K-ras gene mutation. K-ras may promote the overexpression of Fascin-1 by virtue of nuclear expression ofβ-catenin, which provided a new research direction on the treatment of K-ras gene mutated colorectal adenocarcinoma.关键词
结直肠肿瘤/K-ras/Fascin-1/β-catenin/免疫组织化学Key words
colorectal neoplasm/K-ras/Fascin-1/β-catenin/immunohistochemistry分类
医药卫生引用本文复制引用
马桂,王兰,马淑萍,边芳,任艳妮,胡清荣,杨荣..结直肠腺癌中Fascin-1、β-catenin蛋白表达及其与K-ras基因突变状态的相关性[J].临床与实验病理学杂志,2019,35(1):27-32,6.基金项目
甘肃省卫生行业科研计划(GSWSKY-2015-32) （GSWSKY-2015-32）
