临床与实验病理学杂志2019，Vol.35Issue(1)：38-42,5.DOI:10.13315/j.cnki.cjcep.2019.01.009
下调miR-92a对非小细胞肺癌细胞增殖及血管生成因子表达的影响
Effect of up-regulation of miR-92a on the proliferation and angiogenesis of non-small cell lung cancer
摘要
Abstract
Purpose To investigate the effect of down-regulation of miR-92 a on the proliferation and angiogenesis of nonsmall cell lung cancer (NSCLC). Methods Human NSCLC cell A549 was divided into three groups: A549 group (non-transfected A549 cells), sc-siRNA group (A549 cells transfected with sc-siRNA) and miR-92a-siRNA group (A 5 4 9 cells trans-fected with miR-92a-siRNA). The relative expression level of miR-92 a, PTEN and vascular endothelial growth factor (VEGF) in A549 cells and human bronchial epithelial (HBE) cells were detected by RT-PCR and Western blot respectively. The proliferation ability of A549 cells in each group was detected by living cell count and crystal violet staining experiment. Results The relative expression of miR-92 a in A549 cells was significantly higher than that in HBE cells (P < 0.05), the expression level of PTEN protein in A549 cells was significantly lower than that in HBE cells (P < 0.05), and the expression level of VEGF protein was significantly higher than that in HBE cells (P < 0.05).In the miR-92a-siRNA group, the relative expression of miR-92 a decreased (P < 0.05), the expression level of PTEN protein in-creased (P < 0.05), and the expression level of VEGF protein decreased (P < 0.05). The expression levels of PI3 K and Akt in miR-92a-siRNA group decreased (P < 0.05). the number of cells and cell proliferation ability in miR-92a-siRNA group reduced. Conclusion The expression of miR-92 a in NSCLC A549 cells is up-regulated, miR-92 a gene silencing can significantly inhibit cell proliferation and inhibit cell angiogenesis, PTEN and VEGF related PI3K/Akt signaling pathways may play an important role in this process.关键词
肺肿瘤/非小细胞肺癌/A549细胞/人支气管上皮细胞/miR-92a/细胞增殖/血管生成Key words
lung neoplasm/non-small cell lung cancer/A549 cells/human bronchial epithelial cells/miR-92a/cell proliferation/angiogenesis分类
医药卫生引用本文复制引用
汤建华,冯平,张志华,王布..下调miR-92a对非小细胞肺癌细胞增殖及血管生成因子表达的影响[J].临床与实验病理学杂志,2019,35(1):38-42,5.基金项目
河北省医学科学研究重点课题资助计划(20170185) （20170185）
