临床与实验病理学杂志2019，Vol.35Issue(2)：133-138,6.DOI:10.13315/j.cnki.cjcep.2019.02.002
食管鳞状细胞癌中miR-4687-5P及STIM1的表达及调控机制
Expression of miR-4687-5P and STIM1 in esophageal squamous cell carcinoma and their regulation mechanism
摘要
Abstract
Purpose To investigate the expression and the methylation status of miR-4687-5P and STIM1 gene in esophageal squamous cell cancer (ESCC) cell lines and ESCC tissue samples,in order to explore the correlation between miR-4687-5P and STIM1 expression,as well as whether they have a common expression regulation mechanism. Methods The qRTPCR and methylation specific PCR (MSP) methods were applied respectively to examine the expression and methylation of miR-4687-5P and STIM1 genes in ESCC cell lines (TE13, KYSE150,T. Tn) and ESCC samples,and further to analyze their correlation. Results The expression of miR-4687-5P and STIM1 genes in ESCC was significantly decreased,and consistent. The weak expression of miR-4687-5P and STIM1 genes was detected in three ESCC cell lines. After treated with 5-Aza-2'-deoxycytidine (5-Aza-Dc,a demethylation agent) ,the expression levels of these two genes were obviously increased. Meanwhile, the methylation bands were obviously weakened or disappeared. The promoter region of STIM1 gene was hypermethylated in ESCC tissues,and its methylation frequency was correlated with the expression of STIM1 and miR-4687-5P (P < 0. 01) . Conclusion miR-4687-5P and STIM1 genes are down-regulated in esophageal carcinoma,and the expression of miR-4687-5P may be regulated by the promoter of its host gene STIM1,and the hypermethylation may be one of the common mechanisms leading to down-regulatory expression of miR-4687-5P and STIM1 genes in ESCC.关键词
食管肿瘤/鳞状细胞癌/miR-4687-5P/STIM1基因/甲基化Key words
esophageal neoplasms/ esophageal squamous cell cancer/ miR-4687-5P/ STIM1 gene/ methylation分类
医药卫生引用本文复制引用
郭艳丽,牛云峰,杨柳,邝钢,郭炜,董稚明,沈素朋,梁佳..食管鳞状细胞癌中miR-4687-5P及STIM1的表达及调控机制[J].临床与实验病理学杂志,2019,35(2):133-138,6.基金项目
国家自然科学基金(81472335) 、河北省自然科学基金(H2013206315) 、河北省医学科学研究重点课题计划项目(20170698) （81472335）
