临床与实验病理学杂志2019，Vol.35Issue(2)：155-160,6.DOI:10.13315/j.cnki.cjcep.2019.02.007
胃癌组织中piR-9994的表达及其与PIWIL4表达的相关性
Expression of piR-9994 in gastric carcinoma and its correlation with PIWIL4
摘要
Abstract
Purpose To explore the relationship between the expression level of piR-9994 in gastric cancer and its clinical pathological features,and to analyze its correlation with PIWIL4 expression. Methods Express of piR-9994 and PIWIL4 in 76 cases of human gastric cancer tissue with different clinical stage and differentiated degree and matching adjacent tissue were detected by qRT-PCR and immunohistochemistry,respectively. Results The expression of piR-9994 in gastric cancer was 2. 3 times higher than that in paracancerous tissues (P = 0. 002 2) . The expression of piR-9994 in stage Ⅲ + Ⅳ gastric cancer was 3. 5 times higher than that in stage Ⅰ + Ⅱ (P = 0. 002) ,and the expression of piR-9994 in cancers with nerve invasion was 2. 5 times higher than that in cancers without invasion (P = 0. 036) . The positive expression of PIWIL4 in gastric cancer tissues was significantly higher than that in adjacent tissues (χ2 = 18. 346,P < 0. 001) ,and the expression of PIWIL 4 in stage Ⅲ +Ⅳ gastric cancer group was higher than that stage Ⅰ + Ⅱ gastric cancer group (χ2 = 8. 60,P = 0. 003) . There was a positive correlation between piR-9994 expression and PIWIL4 expression in gastric carcinoma (r = 0. 231,P < 0. 05) . Conclusion piR-9994 overexpression in gastric cancer tissues is closely related to tumor staging and nerve invasion,and piR-9994 may promote the occurrence and progression of gastric cancer by regulating PIWIL4 expression. piR-9994 may be a molecular markers for judging malignant degree and prognosis of gastric cancer.关键词
胃肿瘤/piR-9994/PIWIL4/免疫组织化学/qRT-PCRKey words
gastric neoplasms/ piR-9994/ PIWIL4/ immunohistochemistry/qRT-PCR分类
医药卫生引用本文复制引用
夏言,林贤东,胡丹,张和军,林洁琼,陈刚,郑雄伟..胃癌组织中piR-9994的表达及其与PIWIL4表达的相关性[J].临床与实验病理学杂志,2019,35(2):155-160,6.基金项目
福建省卫计委创新课题(2015-CX-7) 、福建省科技厅面上项目(2016J01503) 、福建省卫生教育联合攻关项目(WKJ2016-2-05) 、福建省卫生系统中青年骨干人才培养项目(2015-ZQN-JC-7) 、福建医科大学科技创新联合基金(2017Y9082) （2015-CX-7）
