临床与实验病理学杂志2019，Vol.35Issue(2)：182-186,5.DOI:10.13315/j.cnki.cjcep.2019.02.013
长链非编码RNA-AP000344.3对人膀胱癌细胞增殖和侵袭的影响及机制
Effect of long-chain non-coding RNA AP000344. 3 on the proliferation and invasion of human bladder cancer cells and its mechanism
沈旭 1朱文 1王广 1李建新 1邓全红1
作者信息
- 1. 湖北省荆门市第二人民医院泌尿外科,荆门 448000
- 折叠
摘要
Abstract
Purpose To investigate the effect of specific long-chain non-coding RNA AP000344. 3 on the proliferation and invasion of bladder cancer cells and its mechanism. Methods qRT-PCR was used to detect the expression of AP000344. 3 in bladder cancer cells and normal bladder epithelial cells. The cancer cells with the lowest expression rate were selected for subsequent experiments. The AP000344. 3 plasmid or the negative control plasmid was transferred into bladder cancer cells by Lipofectamine 2000, and the transfection efficiency of AP000344. 3 was detected by qRT-PCR. Cell proliferation activity was measured by MTT method,and cell invasion ability was detected by Transwell assay. Bioinformatics was used to predict downstream miRNA and downstream genes of AP000344. 3. qRT-PCR and Western blot were used to detect the expression of downstream genes. Results The expression of AP000344. 3 in bladder cancer cells was significantly lower than that in normal bladder epithelial cells (P < 0. 01) ,and the expression of AP000344. 3 was the lowest in BIU87 cells (P < 0. 01) . The expression of AP000344. 3 in BIU87 cells was up-regulated at 48 h after transfection with AP000344. 3 (P < 0. 01) . The proliferation activity of BIU87 cells was decreased (P < 0. 05) ,and the cell invasion ability was decreased (P < 0. 05) . AP000344. 3 can target and bind to miR-135a-5p,and miR-135a-5p to human chemokine-like factor superfamily member 3 (CMTM3) . After up-regulation of AP000344. 3,miR-135a-5p expression was down-regulated (P < 0. 01) ,and CMTM3 was up-regulated in mRNA and protein expression (P < 0. 01) . Conclusion AP000344. 3 is significantly down-regulated in bladder cancer cells,and up-regulation of AP000344. 3 can inhibit the proliferation and invasion of bladder cancer cells. The mechanism may be to inhibit the expression of miR-135a-5p and up-regulate the expression of CMTM3 protein,providing a theoretical basis for finding new therapeutic targets for bladder cancer.关键词
膀胱肿瘤/细胞增殖/细胞侵袭/长链非编码RNAKey words
bladder neoplasms/ cell proliferation/ cell invasion/ long-chain non-coding RNA分类
医药卫生引用本文复制引用
沈旭,朱文,王广,李建新,邓全红..长链非编码RNA-AP000344.3对人膀胱癌细胞增殖和侵袭的影响及机制[J].临床与实验病理学杂志,2019,35(2):182-186,5.
