林业科学2019，Vol.55Issue(3)：22-35,14.DOI:10.11707/j.1001-7488.20190303
不同种源北美乔柏和北美香柏幼苗叶挥发性成分的比较
Comparison of Chemical Constituents of Volatile Compounds in Thuja plicata and Thuja occidentalis Seedlings Leaves of Different Provenances
摘要
Abstract
[Objective]To provide a theoretical basis for further development and utilization of the Thuja's resources, the composition and relative content of chemical constituents of essential oils from leaves of Thuja plicata and Thuja occidentalis from different provenances were analyzed.[Method]The healthy 2-year-old seedlings with same size and consistent growth were selected as study materials. The volatile components of new leavesin the same position of seedlings from different provenances were extracted by solid-phase micro-extraction (SPME), and separated and identified by gas chromatography-mass spectrometry (GC-MS). Qualitative analysis was carried out by mass spectrometry and the relative content of volatile components was calculatedby peak area normalization.[Result]A total of 156 chemical components of essential oils of T.plicata and T.occdentalis were identified, among which 74 components were identified in T.plicata, and95 components in T.occdentalis. The volatile components of the two species from 10 provenances were dominated by monoterpenes, and the average relative content of T.plicata (80.258％) was higher than that of T.occdentalis (69.445％), but not forthe sesquiterpenoidsand other terpenoids.The two specieswere significantly differentin the composition of the essential oil. For example, 27 components were uniquely obtainedfrom 5 T.plicata provenancesand 35 components from 5 T.occdentalis provenances. There were only 6 common components in 10 provenances and their contents were significantly different The common components wereα-Thujone, γ-Terpinene, (8β, 13β) -13-methyl-17-Norkaur-15-ene, α, α-4-trimethyl-3-cyclohexene-1-methanol acetate, Caryophyllene and Hexadecanoic acidmethyl ester, respectively.The main components ofthe essential oildiffered obviously betweentwo species. The same main componentswere only thujone and β-phellandrene whichcontents were the highestinT.plicata and T.occdentalis.[Conclusion]The chemical compositionand content of essential oils from leaves of T.plicata and T.occdentalis in different provenances weresignificantlydifferent, but the content of thujone was the highest in both species.关键词
北美香柏/北美乔柏/挥发性成分固相微萃取气相色谱-质谱联用Key words
Thuja plicata/Thuja occidentalis/volatile component/solid phase micro-extraction (SPME)/gas chromatography-mass spectrometry (GC-MS)分类
农业科技引用本文复制引用
倪妍妍,肖文发,刘建锋,张玉婷,胡军..不同种源北美乔柏和北美香柏幼苗叶挥发性成分的比较[J].林业科学,2019,55(3):22-35,14.基金项目
中央级公益性科研院所基本科研业务费专项"崖柏属植物资源收集与评价"(CAFYBB2018SY002) （CAFYBB2018SY002）
国家林业局948项目"崖柏属优良种质资源与培育技术引进"(2013-4-47) （2013-4-47）
