林业科学2019，Vol.55Issue(3)：64-71,8.DOI:10.11707/j.1001-7488.20190307
脱落酸和玉米素结合使用对杂交鹅掌楸体胚分化及发育的影响
Effects of Combined ABA and ZT Treatment on Somatic Embryogenesis and Development of Liriodendron sino-americanum
摘要
Abstract
[Objective] In this article, we aimed to establish large scale propagation methodologies for Liriodendron sino-americanum (hybrid Liriodendron) and to this end we investigated how various combined treatments with the plant hormones abscisic acid (ABA) and zeatin (ZT) affect its somatic embryogenesis and development.[Method] We successfully induced somatic embryos using the embryogenic callus of L. sino-americanum genotype 15-5202. We tested how adding either ABA alone or ABA and ZT together to the differentiation medium affected somatic embryogenesis. Our differentiation medium induced somatic embryos with a high frequency and was screened by counting the number of somatic embryos and observing their growth and development.[Result] 1) The addition of ABA to the medium showed positive effect on the induction and maturation of somatic embryos. 0.5-1.5 mg·L-1 ABA in the medium accelerated the development of somatic embryos with largely normal morphology. When adding 2.0-2.5 mg·L-1 ABA, the differentiation rate of somatic embryos increased significantly, but the proportion of abnormal embryos increased. After 30 days of treatment with low-concentration ABA, somatic embryos matured with an additional 2 weeks of culture in light. By contrast, on medium without ABA, somatic embryos were white and not fully matured with pale yellow cotyledon. 2) Adding ABA together with ZT to the medium significantly increased the differentiation rate and development of somatic embryos. Treatment with 2.0 mg·L-1 ABA combined with 0.2-0.4 mg·L-1 ZT induced somatic embryos with normal morphology, clearly showing green cotyledons and a radicle after 2 weeks light culture, while the growth and development of somatic embryos was noticeably slower without ZT treatment. Adding ZT concentration of higher than 0.6 mg·L-1 inhibited growth and development of somatic embryos and was unfavorable to their induction and maturation.[Conclusion] A differentiation medium containing 2.0 mg·L-1 ABA and 0.2-0.4 mg·L-1 ZT, significantly increases the efficiency of L. sino-americanum somatic embryogenesis and promotes normal development of somatic embryos.关键词
杂交鹅掌楸/体细胞胚胎发生/脱落酸/玉米素Key words
Liriodendron sino-americanum/somatic embryogenesis/ABA/ZT分类
农业科技引用本文复制引用
陈婷婷,王鹏凯,张稼霁,施季森,成铁龙,陈金慧..脱落酸和玉米素结合使用对杂交鹅掌楸体胚分化及发育的影响[J].林业科学,2019,55(3):64-71,8.基金项目
江苏省现代农业重点项目(BE2017376) （BE2017376）
江苏省林业科技创新与推广项目(LYKJ[2017]42) （LYKJ[2017]42）
江苏省青蓝工程项目 （）
南京林业大学南方现代林业协同创新中心 （）
江苏省高校优势学科建设工程资助项目(PAPD) （PAPD）
