西南医科大学学报2019，Vol.42Issue(1)：15-19,5.DOI:10.3969/j.issn.2096-3351.2019.01.003
奥曲肽调控JAK/STAT3/SOCS3信号通路对肝星状细胞相关肝纤维化的影响
Protective effect of octreotide on hepatic stellate cell-related hepatic fibrosis by regulating the JAK/STAT3/SOCS3 signaling pathway
摘要
Abstract
Objective:To investigate the protective effect of octreotide (OCT) on hepatic fibrosis and potential mechanism of related signaling pathway.Methods:Human hepatic stellate LX-2 cells were cultured in vitro and were then treated with OCT at different concentrations (10-3, 10-4, 10-5, and 10-6 mmol/L) for different durations (24, 48, and 72 hours). RT-PCR and Western blot were used to measure the mRNA and protein expression of STAT3, phosphorylated STAT (p-STAT), and SOCS3.Results:Compared with the 24 hour control group, all OCT groups had significant reductions in the mRNA expression of STAT3 (F = 198.37, P = 0.000) and SOCS3 (F = 173.45, P =0.000). Compared with the 48 hour control group, all OCT groups had significant reductions in the mRNA expression of STAT3 (F = 105.79, P = 0.000) and SOCS3 (F = 167.38, P ＜ 0.05). Compared with the 72 hour control group, all OCT groups had significant reductions in the mRNA expression of STAT3 (F = 87.44, P ＜ 0.05) and SOCS3 (F =210.66, P = 0.000). After 24 hours of OCT intervention, all OCT groups had significant reductions in the protein expression of p-STAT3 (F = 112.28, P ＜ 0.05) and SOCS3 (F = 146.82, P = 0.000). Compared with the 48 hours control group, all OCT groups had significant reductions in the protein expression of p-STAT3 (F = 87.34, P ＜ 0.05) and SOCS3 (F = 66.92, P = 0.000). Compared with the 72 hour control group, all OCT groups had significant reductions in the protein expression of p-STAT3 (F = 98.05, P ＜ 0.05) and SOCS3 (F = 227.68, P = 0.000). With drug concentration remaining the same, the expression of SOCS3 decreased over the time of intervention. With the intervention time remaining the same, the expression of STAT3 and p-STAT3 increased with the increase in OCT concentration.Conclusion:The JAK/STAT3 pathway and its negative regulatory factor SOCS3 may be involved in the mechanism of OCT in regulating the proliferation and apoptosis of LX-2 cells. OCT can reduce the mRNA and protein expression of STAT3, p-STAT3, and SOCS3 and thus inhibit hepatic fibrosis.关键词
奥曲肽/人肝星状细胞/JAK/STAT3通路/p-STAT3/SOCS3Key words
Octreotide/Human hepatic stellate cell/JAK/STAT3 pathway/p-STAT3/SOCS3分类
医药卫生引用本文复制引用
蒋亚玲,周贤,刘蔚,陈辉,陈珊珊..奥曲肽调控JAK/STAT3/SOCS3信号通路对肝星状细胞相关肝纤维化的影响[J].西南医科大学学报,2019,42(1):15-19,5.基金项目
泸州市科技局计划项目(2011-I-S37) （2011-I-S37）
