南京林业大学学报（自然科学版）2019，Vol.43Issue(1)：25-31,7.DOI:10.3969/j.issn.1000-2006.201808050
BpMYB4基因在白桦中的遗传转化及低温胁迫应答反应
Functional study of BpMYB4 in birch response to low temperature stress
摘要
Abstract
[Objective]Our objective was to understand the functions of the Bp MYB4 gene during low-temperature stress.[Method]We performed the following experiments in this study.Firstly, we analyzed the expression pattern of the Bp MYB4 gene in different tissues of birch, including roots, stems, leaves, xylem and buds.Secondly, we constructed the plant overexpression vector of PGWB2-Bp MYB4, and generated birch transgenic lines that overexpressed Bp MYB4 using Agrobacterium-mediated transformation.Then, a polymerase chain reaction (PCR) and quantitative real-time PCR (qRT-PCR) were used to identify the transformants.Finally, we examined eight physiological indexes of transgenic birch under low temperature using spectrophotometry.[Result]Campare with the expression of Bp MYB4 in buds, the highest expression level of Bp MYB4 was in the veins of the third leaf and the tender stems between the first leaf and the second leaf.We obtained 13 independent 35S∶∶Bp MYB4 transgenic lines and PCR results provided preliminary evidence that exogenous Bp MYB4 was integrated into the genome of birch.qRT-PCR results indicated that Bp MYB4 expression in MYB-8, MYB-11 and MYB-12 lines was significantly higher than that in the non-transgenic birch (NT).Under cool stress, overexpression of the Bp MYB4 gene in birch increased enzyme activities of SOD, POD and CAT.Overexpression of Bp MYB4 also increased contents of OPC, soluble sugar, soluble protein, and proline.Compared to NT, the content of malondialdehyde (MDA) in transgenic birch was reduced.[Conclusion]The results indicated that Bp MYB4 transgenic birch could produce protective substances under cold stress, and it will have an ability to resist cold.It can be developed into a freeze-resistant preferred strain.关键词
白桦/Bp MYB4/遗传转化/低温胁迫/抗寒Key words
Betula platyphylla Suk./Bp MYB4/genetic transformation/cold stress/cold resistance分类
农业科技引用本文复制引用
张宇,陈肃,高源,黄海娇..BpMYB4基因在白桦中的遗传转化及低温胁迫应答反应[J].南京林业大学学报（自然科学版）,2019,43(1):25-31,7.基金项目
国家自然科学基金项目(31870659) （31870659）
