南京林业大学学报（自然科学版）2019，Vol.43Issue(1)：32-38,7.DOI:10.3969/j.issn.1000-2006.20186025
白桦BpTCP7基因启动子的克隆及表达分析
Cloning and expression analysis of BpTCP7 promoter from Betula platyphylla
摘要
Abstract
[Objective]Bp TCP7 gene, a member of the CIN branch of TCP family, plays an important role in plant growth and development.In order to characterize the function of Bp TCP7.[Method]A 1 791 bp flanking sequence upstream of the translation initiation codon was cloned, and putative cis-regulatory elements were deciphered from the promoter sequence of Bp TCP7 using PLACE and Plant CARE web tools, and transferred into Arabidopsis thaliana.Then, the expression patterns were investigated during salt and drought stress.[Result]The results showed that cis-regulatory elements involved in cell cycle, flowering, leaf development, multiple hormone-responsiveness, and stress response were included in the promoter region.The Gus reporter gene that was drived the Bp TCP7 promoter was detected in all tissues and organs during the reproductive and vegetative phases.Notably, the Bp TCP7 promoter was expressed in the leaf margin of young and mature leaves.The expression level of Bp TCP7 gene was increased in transgenic A.thaliana after Na Cl and polyethylene glycol (PEG) stresses compared with the control.[Conclusion]In conclusion, Bp TCP7 participates in biological processes of leaf development, hormone response, drought and salt stress response, and to some extent regulates the development of all tissues and organs during the reproductive and vegetative growth phases.关键词
白桦/Bp TCP7启动子/顺式作用元件/克隆/表达分析Key words
Betula platyphylla/Bp TCP7 promoter/cis-elements analysis/clone/expression analysis分类
农业科技引用本文复制引用
任丽,董京祥,杨洋,黄海娇,李慧玉..白桦BpTCP7基因启动子的克隆及表达分析[J].南京林业大学学报（自然科学版）,2019,43(1):32-38,7.基金项目
国家重点研发计划(2017YFD0600603) （2017YFD0600603）
