南京林业大学学报（自然科学版）2019，Vol.43Issue(1)：76-82,7.DOI:10.3969/j.issn.1000-2006.201804003
一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性
Identification and characteristics of a strain of Serratia marcescens isolated from the termites, Odontotermes formosanus
摘要
Abstract
[Objective]With increasing attention on the ecological environment, biological control of termites needs to be developed.[Method]A strain of a bacteria producing red pigment was screened from Odontotermes formosanus.After being separated and purified, a purer strain was obtained and named SM1.Then, SM1 was inoculated into O.formosanus, which caused the healthy termites to turn red and die.The bacteria was identified based on its morphological ch-aracteristics, physiological and biochemical characteristics, red pigment, and 16S r DNA.[Result]The bacterial colony was round, easy to pick up, had slight odor, was smooth and sticky, and had a diameter of 1-3 mm and a middle vermilion that was not transparent.In addition, ten physiological and biochemical characteristics of the SM1 strain were measured.These results showed that the bacterium was Serratia marcescens.Furthermore, the size of its cloned16S r DNA fragment was 1 418 bp, and the homology with Serratia marcescens was 99％.Therefore, the bacteria was determined to be Serratia marcescens.Further, a higher purity of red pigment could be obtained by ultrasonic-assisted acetone extraction, and the pigment was dissolved in ethanol solution and then analyzed by ultraviolet full wavelength scanning.The results showed that the pigment had an obvious absorption peak at 535 nm, which suggested that the red pigment was a prodigiosin.[Conclusion]The bacterium screened from Odontotermes formosanus was Serratia marcescens, which created a theoretical foundation for the biological control of termites.关键词
白蚁/生理生化/16S rDNA/粘质沙雷氏菌/灵菌红素Key words
termite/physiological and biochemical characters/16S rDNA/Serratia marcescens/prodigiosin分类
农业科技引用本文复制引用
傅仁杰,祁雪连,丰凯,夏馨蕊,汤方..一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性[J].南京林业大学学报（自然科学版）,2019,43(1):76-82,7.基金项目
江苏省自然科学基金项目(BK20151517) （BK20151517）
镇江市重点研发计划-现代农业(NY2017002) （NY2017002）
句容市农业科技支撑计划(NY2017846771) （NY2017846771）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
江苏省高等学校大学生创新创业训练计划重点项目(201610298041Z) （201610298041Z）
