热带亚热带植物学报2019，Vol.27Issue(1)：60-64,5.DOI:10.11926/jtsb.3935
催吐萝芙木离体培养和植株再生体系的建立
In vitro Culture and Establishment of Rapid Regeneration System of Rauvolfia vomitoria
摘要
Abstract
To constructe the rapid regeneration system of Rauvolfia vomitoria Afzel., the effects of plant growth regulators on callus induction, differentiation, and rooting were studied with young stems as explants. The results showed that the optimal medium for callus induction was MS+2, 4-D 1.0 mg L–1+TDZ 0.5 mg L–1 or MS+2, 4-D 2.0 mg L–1+TDZ 0.5 mg L–1 with callus induction rate of 100％. The optimal medium for callus differentiation was MS+6-BA 3.0 mg L–1+NAA 0.1 mg L–1 with shooting rate of 46.6％ and average buds of 3.04. So, these would lay a foundation for studying on rapid regeneration and genetic transformation of R. vomitoria.关键词
催吐萝芙木/愈伤组织/再生/组织培养Key words
Rauvolfia vomitoria Afzel./Callus/Regeneration/Tissue culture引用本文复制引用
董佳颖,杨小田,向小群,郭晋雅,高峰..催吐萝芙木离体培养和植株再生体系的建立[J].热带亚热带植物学报,2019,27(1):60-64,5.基金项目
国家自然科学基金项目(31171601)资助 （31171601）
