热带亚热带植物学报2019，Vol.27Issue(1)：109-114,6.DOI:10.11926/jtsb.3901
液体培养的蛇足石杉离体叶状体生产石杉碱甲和生化特征研究
Studies on Huperzine A Accumulation and Biochemical Characteristics in Thallus of Huperzia serrata in vitro by Liquid Culture
摘要
Abstract
In order to understand the effect of liquid cultures on thallus of Huperzia serrate (Thunb.) Trev. in vitro, the changes in thallus growth, huperzine A accumulation, antioxidant enzymes activities and endogenous hormones were studied cultured on different dosage of liquid medium. The results showed that the huperzine A contents had significant differences among different dosages of liquid medium. The huperzine A content could reach up to 3 115.159 μg L–1 with 15 mL medium, which was 2.20 times more than the control. The superoxide dismutase activity of thallus cultured in shallow liquid was higher than that of the control, the activity of peroxidase was higher than that of the control at early stage, while did the activity of catalase at later stage. The contents of endogenous hormones IAA and GA were lower than the control, while ABA content was higher than the control. Therefore, it was suggested that the ability to produce HupA was high in thallus in vitro of H. serrata cultured with shallow liquid medium, the HupA content had relation with the content of endogenous hormone ABA, and the activities of three antioxidant enzymes in thallus had collaborative enhancement effect during growth stage.关键词
蛇足石杉/离体叶状体/石杉碱甲/液体培养/抗氧化酶/内源激素Key words
Huperzia serrate/Thallus in vitro/Huperzine A/Liquid culture/Antioxidant enzyme/Endogenous hormone引用本文复制引用
袁慧慧,涂艺声,黄倩,余晓..液体培养的蛇足石杉离体叶状体生产石杉碱甲和生化特征研究[J].热带亚热带植物学报,2019,27(1):109-114,6.基金项目
国家自然科学基金项目(81360614,81660597) （81360614,81660597）
江西省自然科学基金项目(20132BAB204023)资助 （20132BAB204023）
