四川中医2019，Vol.37Issue(4)：57-60,4.
苦参碱对视网膜母细胞瘤细胞增殖与凋亡及PI3K/Akt信号通路的影响
Effects of Matrine on Proliferation, Apoptosis and PI3K/Akt Signaling Pathway for Retinoblastoma Cells
摘要
Abstract
Objective:To investigate the effect of Matrine on proliferation, apoptosis and PI3 K/Akt signaling pathway for retinoblastoma cells.Methods:The Rb cell line Y79 were cultured, using different concentrations (15, 30, 60, 120 mg/L) of matrine were cultured as the observation group of cells, and the blank group as the controlled group, MTT method (MTT) to detect growth inhibition, cell apoptosis by flow cytometry.The apoptosis rate was calculated by RT-PCR detection of PI3 K, the relative expression of AktmRNA and PI3 K was detected by immunohistochemical method, the expression of Akt protein.Results:After culturing 24 h, 48 h and 72 h, the growth inhibition rate of Y79 cells in the observation group was higher, and the growth inhibition rate was also increased with the increase of time and matrine concentration (P ＜ 0.05).After training, the proportion of cells in the observation group in GO/G1 phase was higher, and the cells in phase S and G2/M phase were lower (P ＜ 0.05).At the same time, with the increase of matrine concentration, the apoptosis rate increased significantly and higher (P ＜ 0.05).The relative expressions of PI3 KmRNA and AktmRNA in the observation group were significantly lower, and with the increase of concentration, the relative expression of PI3 KmRNA and AktmRNA decreased significantly (P ＜ 0.05).In the observation group, the expressions of PI3 K and Akt proteins were lower, and with the increase of concentration, the expression of PI3 K and Akt protein also declined (P ＜ 0.05).Conclusion:Matrine can significantly inhibit the proliferation of Rb cells and promote apoptosis, which may cause the cells to stay in the GO/G1 phase and induce apoptosis by inhibiting the PI3 K/Akt signaling pathway.关键词
视网膜母细胞瘤/PI3K/Akt信号通路/苦参碱/细胞增殖/细胞凋亡Key words
Retinoblastoma/PI3K/Akt signaling pathway/Matrine/Cell proliferation/Apoptosis分类
医药卫生引用本文复制引用
张铮..苦参碱对视网膜母细胞瘤细胞增殖与凋亡及PI3K/Akt信号通路的影响[J].四川中医,2019,37(4):57-60,4.基金项目
承德市科学技术研究与发展计划(编号:201606A062) （编号:201606A062）
