山东医药2018，Vol.58Issue(48)：9-12,28,5.DOI:10.3969/j.issn.1002-266X.2018.48.003
重症肌无力患者血清microRNA-146a水平变化及其生物信息学分析
Changes and bioinformatics analysis of microrna-146a in serum of patients with myasthenia gravis
摘要
Abstract
Objective To observe the changes in levels of microRNA-146a in the serum of patients with myasthenia gravis (MG), and to analyze the molecular regulatory network of microRNA-146a in the pathogenesis of MG by bioinformatics.Methods One hundred and eight patients who was first diagnosed MG (MG group), and 50 healthy persons in the same period (control group) were selected, and then the serum microRNA-146a was tested by RT-PCR.The UCSC genome online tools were used to analyze the position and conservatism of microRNA-146a in the human genome.Targetscan and Co MeTa database was used to predict the inter-target gene of microRNA-146a.DAVID database was used to analyse the functional enrichment (GO enrichment) and signal pathway enrichment (KEGG Pathway) of target gene in microRNA-146a.Results The serum microRNA-146a in the MG group was 2.13±0.63, which was higher than that (0.86±0.38) of control group (P＜0.05).Nucleotide sequence was highly conserved in microRNA-146a, with 88 types of potential target genes ERBB4, NUMB, CD80, TRAF6, IRAK1, etc.GO analysis showed that the function of microRNA-146a target gene mainly focused on the synthesis process of cell proliferation regulation, neuronal differentiation, phosphorus metabolism reaction, immune inflammation, and cytokines, etc.KEGG Pathway showed that microRNA-146a target gene mainly focused on Toll receptor signaling pathway, neurotransmitter regulatory signaling pathway, EB signaling pathway, and other signaling pathways.Conclusion The level of microRNA-146a is elevated in the serum of MG patients, which can participate in the pathogenesis of MG by acting on multiple signaling pathways.关键词
重症肌无力/微小RNA-146a/生物信息学分析/靶基因/功能富集/信号通路富集Key words
myasthenia gravis/microRNA-146a/bioinformatics analysis/target gene/functional enrichment/signal pathway enrichment分类
医药卫生引用本文复制引用
黄攀,徐敏,何晓英,王淳..重症肌无力患者血清microRNA-146a水平变化及其生物信息学分析[J].山东医药,2018,58(48):9-12,28,5.基金项目
四川省应用基础研究计划项目 (2014JC0166) （2014JC0166）
四川省卫生厅科研课题 (110368) （110368）
