山东医药2018，Vol.58Issue(48)：13-16,4.DOI:10.3969/j.issn.1002-266X.2018.48.004
生物钟基因在腹部恶性肿瘤组织中的表达差异
Expression of circadian clock genes in abdominal malignant tumor
摘要
Abstract
Objective To explore the expression differences of clock genes in the abdominal malignant tumors.Methods The RNA-Seq expression of 14 kinds of clock genes in six abdominal malignant tumors and adjacent normal tissues (liver, gastric, pancreatic, kidney, rectal, and colon cancer) from the Cancer Genome Atlas (TCGA) database was integrated and evaluated to observe the differential expression of clock genes.We used the software edgeR in Bioconductor to standardize the RNA-Seq data.ResultsFourteen clock genes were significantly lowly expressed in the cancer tissues of six abdominal malignant tumors.Compared with the adjacent normal tissues, the expression levels of Cry2, CLOCK, Timeless, NPAS2, NR1D1, DEC1-2, and RORA were significantly lower in the liver cancer tissues (all P＜0.05).Compared with the adjacent normal tissues, the expression levels of Per1-2, Cry1-2, CLOCK, Timeless, NPAS2, NR1D1-2, DEC2, and RORA were significantly lower in the kidney cancer tissues (all P＜0.05).Compared with adjacent normal tissues, the expression levels of Per1-3, Cry1-2, Timeless, BMAL1, NR1D2, DEC1-2, and RORA were significantly lower in the colon cancer tissues (all P＜0.05).Compared with adjacent normal tissues, the expression levels of Per1, Per3, Cry1-2, CLOCK, BMAL1, NPAS2, NR1D1, DEC2, and RORA were significantly lower in rectal cancer tissues (all P＜0.05).Compared with the adjacent normal tissues, the expression levels of Per1, Per3, Cry2, CLOCK, Timeless, BMAL1, and NPAS2 were significantly lower in the gastric cancer tissues (all P＜0.05).ConclusionDifferent clock genes are expressed differently in different abdominal malignant tumors.关键词
腹部恶性肿瘤/生物钟基因/昼夜节律/癌症基因组图谱数据库Key words
abdominal malignant neoplasm/clock gene/circadian rhythm/cancer genome atlas database分类
医药卫生引用本文复制引用
邱梦君,熊枝繁,何晓晓,毕柠瑞,汪朦朦,杨盛力..生物钟基因在腹部恶性肿瘤组织中的表达差异[J].山东医药,2018,58(48):13-16,4.基金项目
国家自然科学基金资助项目 (81702885) （81702885）
湖北省自然科学基金资助项目 (2017CFB178) （2017CFB178）
中央高校基本科研业务费专项资金项目 (0118530328) （0118530328）
中国肝炎防治基金会-天晴肝病研究基金科研课题 (TQGB20170005) （TQGB20170005）
