山东医药2018，Vol.58Issue(48)：25-28,4.DOI:10.3969/j.issn.1002-266X.2018.48.007
微小RNA-17在幽门螺杆菌相关胃癌组织中的表达变化及意义
Expression and significance of microRNA-17 in Helicobacter pylori-associated gastric cancer
摘要
Abstract
Objective To observe the expression change of microRNA-17 in the helicobacter pylori (Hp) -associated gastric cancer tissues and its clinical significance.MethodsSeventy-five ceases of gastric cancer tissue specimens and their matched adjacent tissue specimens from patients with gastric cancer were collected, including 48 patients with hp-positive gastric cancer and 27 patients with hp-negative gastric cancer.Real-time quantitative PCR was used to determine the expression levels of cytotoxin-related gene A (Cag A) mRNA and miR-17.Results The expression levels of Cag A mRNA in Hp-positive gastric cancer tissues (6.061±0.712) was significantly higher than that in the Hp-negative gastric cancer patients (1.024±0.235) (t=35.593, P＜0.05).The miR-17 expression levels of gastric cancer tissues and adjacent tissues in patients with Hp-positive gastric cancer were significantly higher than those in patients with Hp-negative gastric cancer (12.785±1.324 vs 5.654±0.745, 8.124±1.105 vs 3.124±0.564) (t=23.739, 21.911, P＜0.01).The miR-17 expression level of gastric cancer tissues in the Hp-positive and Hp-negative patients was significantly higher than that in the adjacent tissues (12.785±1.324 vs 8.124±1.105, 5.654±0.745 vs 3.124±0.564) (t=18.725, 14.069, P＜0.01).In the gastric cancer tissues, miR-17 expression was positively correlated with Cag A expression (r=0.564, P＜0.01).The level of miR-17 expression of gastric cancer in patients with tumor diameter>5cm, TNM stagingⅢ-Ⅳ, Borrmann typeⅢ-Ⅳand lymph node metastasis Hp infection was significantly higher than that of patients with tumor diameter less≤5 cm, TNM stageⅠ-Ⅱ, Borrmann typing-Ⅱ, non-lymph node metastasis (15.226±1.325 vs 11.041±1.214, 14.485±1.412 vs 11.571±1.1.16, 14.341±1.325 vs 11.468±1.241, 15.124±1.332 vs 9.778±1.123), with statistically significant difference (t=11.336, 7.983, 7.748, and 7.748, all P＜0.05).Conclusion The abnormal expression of miR-17 in gastric cancer tissues with Hp infection is significantly related to tumor diameter, TNM staging, Borrmann typing, lymph node metastasis, and prognosis, which can be used as a candidate marker for early diagnosis and prognosis of gastric cancer.关键词
胃癌/幽门螺杆菌/微小RNA-17/细胞毒素相关基因AKey words
gastric carcinoma/Helicobacter pylori/microRNA-375/cytotoxin-related gene A分类
医药卫生引用本文复制引用
朱艳丽,杜井峰,苏德望,刘亮,程卓鑫,张雪梅..微小RNA-17在幽门螺杆菌相关胃癌组织中的表达变化及意义[J].山东医药,2018,58(48):25-28,4.基金项目
黑龙江省卫生计生委科研课题 (2016-303, 2017-384) （2016-303, 2017-384）
