摘要
Abstract
Objective To observe the changes of miR-21 induced by lipopolysaccharide (LPS) in the inflammatory reaction of human renal cortical proximal tubular epithelial cells (HK-2 cells), and to explore the role of miR-21 by transfecting miR-21 mimics (miR-21 mimics).Methods HK-2 cells were cultured and we induced its inflammatory response by LPS.HK-2 cells were cultured, then transfected with miR-21 mimics or NC, and and we induced its inflammatory response by LPS.The cells were collected, and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect miR-21 and IL-6 mRNA, and Western blotting was used to detect IL-6 protein.Results (1) Compared with the control group, the mRNA and protein expression of IL-6 increased at each time point in the LPS-stimulated HK-2cells (P＜0.05), and the miR-21 expression decreased (P＜0.05). (2) Compared with the cells transfected with NC mimics, the expression of miR-21 increased in HK-2 cells after transfection with miR-21 mimics for 24 h (P＜0.05).After transfection with miR-21 mimics for 24 h and 10 ug/m L LPS stimulation for 8 h, the expression of IL-6 mRNA decreased in HK-2 cells as compared with that of HK-2 cells undergoing NC mimics transfection and LPS stimulation (P＜0.05). (3) After transfection with miR-21 mimics for 24 h and LPS treatment for 12 h, the expression of miR-21 increased in HK-2 cells as compared with that of HK-2 cells undergoing NC mimics transfection and LPS stimulation (P＜0.05);after transfection with miR-21 mimics for 24 h and LPS treatment for 24 h, IL-6 protein expression decreased in HK-2 cells as compared with that of HK-2 cells undergoing NC mimics transfection and LPS stimulation of 24 h (P＜0.05).ConclusionLPS can induce inflammatory response in HK-2 cells, IL-6 expression increases, and miR-21 expression decreases.Transfection with miR-21 mimics can attenuate LPS-induced inflammatory response in HK-2 cells.关键词
脓毒症/急性肾损伤/微小RNA-21/脂多糖/炎症反应Key words
sepsis/acute kidney injury/miRNA-21/lipopolysaccharide/inflammatory response分类
医药卫生
