山东医药2018，Vol.58Issue(48)：33-36,4.DOI:10.3969/j.issn.1002-266X.2018.48.009
原花青素对尿路致病性大肠杆菌黏附、侵袭膀胱上皮细胞的影响及其机制
Effects of procyanidin on adhesion and invasion of uropathogenic escherichia coli to bladder epithelial cells
摘要
Abstract
Objective To investigate the effect and mechanism of procyanidin on the adhesion and invasion of uropathogenic Escherichia coli (UPEC) to bladder epithelial cells.Methods Human bladder epithelial cells T24 were cultured in vitro and randomly divided into the blank control group, negative control group, and procyanidin group.The cells in the negative control group and the procyanidin group were respectively added with equal volume of DMSO and procyanidin solution with antibacterial concentration.The relative adhesion and invasion rates of UPEC J96 were detected by plate colony counting method.RT-PCR was used to detect the mRNA expression of integrinα3 andβ1 on the cell surface of each group.The relative fluorescence intensity of fibrin F-Actin of each group was observed by flow cytometry.Results Compared with the blank control group, the adhesion rate and invasion rate of J96 in the procyanidin group decreased, with statistically significant difference (both P＜0.01).The mRNA expression levels of integrinα3 andβ1 on the cell surface of T24 cells decreased, with statistically significant differences (P＜0.01).Compared with the blank control group, the relative fluorescence intensity of F-Actin decreased in the proanthocyanidin group, whether they were UPEC J96 uninfected cells or UPEC J96 infected cells and the difference was statistically significant (P＜0.01).Conclusion Procyanidin can inhibit the adhesion and invasion of UPEC to bladder epithelial cells by inhibiting the expression of integrin receptor and F-Actin on the surface of bladder epithelial cells.关键词
尿路感染/原花青素/尿路致病性大肠杆菌/肌动蛋白聚合/整合素受体/膀胱上皮细胞Key words
urinary tract infection/procyanidin/uropathogenic Escherichia coli/actin polymerization/integrin receptor/bladder epithelial cells分类
医药卫生引用本文复制引用
沙凯辉,刘同刚,张晓丽..原花青素对尿路致病性大肠杆菌黏附、侵袭膀胱上皮细胞的影响及其机制[J].山东医药,2018,58(48):33-36,4.基金项目
山东省医药卫生科技发展计划项目 (2017WS886) （2017WS886）
