刘泓键 1奉友刚 1余周 1康永明 1贺炜 1姜明东1
作者信息
- 1. 遂宁市中心医院, 四川遂宁 629000
- 折叠
摘要
Abstract
ObjectiveTo observe the effects of silencing long-chain non-coding RNA (LncRNA) HOTAIR on the proliferation and apoptosis of bladder cancer cell line 253J and to explore its mechanism.Methods 253J cells in logarithmic phase were divided into the si HOTAIR interference group, empty vector group, and control group.The cells in the si HOTAIR interference group and empty vector group were transfected with si HOTAIR (which could silence the expression of HOTAIR) and the negative sequence of the empty vector, respectively.No treatment was done in the control group.CCK8assay was used to observe the proliferation of cells in each group after 4-hour transfection, and Hoechst method was used to observe the apoptosis in each group.The mRNA expression of Notch1, Ep CAM, CXCR2, E-cadherin, vimentin, matrix metalloproteinase 2 (MMP-2) andα-SMA in each group was detected by real-time fluorescence quantitative PCR.ResultsAt 4 h after transfection, the OD values of the si HOTAIR interference group, empty vector group and control group were0.38±0.02, 0.89±0.01, and 0.89±0.02, respectively.Compared with the empty vector group and control group, the OD value of the si HOTAIR group decreased (P＜0.05).At 4 h after transfection, the number of apoptotic cells in the si HOTAIR interference group, empty vector group and control group were 33.56±2.26, 13.69±1.25, and 12.85±1.35, respectively.The number of apoptotic cells in the si HOTAIR group was higher than that of the control group and empty vector group (P＜0.05).Compared with the control group and empty vector group, the relative mRNA expression of Notch1, Ep CAM, CXCR2, Vimentin, MMP-2, andα-SMA decreased, but E-cadherin mRNA expression increased in the si HOTAIR interference group (all P＜0.05).ConclusionSilencing HOTAIR expression can inhibit the proliferation and promote the apoptosis of bladder cancer cells by inhibiting the expression of Notch1, Ep CAM, CXCR2, Vimentin, MMP-2, andα-SMA and promoting the expression of E-cadherin.关键词
膀胱癌/长链非编码RNA HOTAIR/细胞增殖/细胞凋亡/作用机制Key words
bladder carcinoma/long-chain non-coding RNA HOTAIR/cell proliferation/apoptosis/mechanism of action分类
医药卫生
