山东医药2018，Vol.58Issue(48)：45-48,4.DOI:10.3969/j.issn.1002-266X.2018.48.012
生脉散对2型糖尿病大鼠胰岛β细胞NF-κB/IκB及VEGF表达的影响
Effect of Shengmai power on VEGF expression of pancreatic β cells in type 2 diabetic rats
摘要
Abstract
Objective To observe the regulating effect of Shengmai powder on the expression levels of nuclear factor-κB/inhibitor nuclear factor-κB (NF-κB/IκB) and vascular endothelial growth factor (VEGF) in the pancreaticβcells of rats with type 2 diabetes mellitus (T2DM).Methods Totally 120 SD male rats were randomly divided into the groups A, B, C, D, E, and F.Rats in the group A were given basic diet, while rats in the other groups (B, C, D, E and F) were fed with high-fat diet combined with intraperitoneal injection of low-dose streptozotocin (STZ) to establish the rat models of diabetic obesity.After 8 weeks, the successfully established rat models were selected from each group.Rats in the group B were given the same amount of normal saline by gavage, group C 0.2 g/ (kg·d) aspirin, and groups D, E and F were given 4.7, 9.4 and 18.8 g/ (kg·d) Shengmai powder.All treatments lasted for 4 weeks.Fasting blood glucose (FBG), fasting insulin (FINS), insulin sensitivity index (ISI) and insulin resistance (HOMA-IR) were measured before and after the above intervention.In addition, serum VEGF was detected by enzyme-linked immunosorbent assay (ELASA), the change of vascular wall structure was observed by HE staining, and NF-κB and IκB mRNA in isletβcells of rats were detected by real-time PCR.Results After 4 weeks of treatment, HE staining showed that the change of vascular wall structure was ameliorated and accompanied with tendency of repair in the groups D, E and F, especially in the latter two groups (P＜0.05).Compared with the group B, the levels of FBG, FINS, and HOMA-IR decreased in the groups D, E and F, while the level of ISI increased.Compared with the group C, the levels of FBG and FINS were higher in the group D, the levels of FBG, FINS, and HOMA-IR were lower, and the level of ISI was higher in the groups E and F, with statistically significant difference (all P＜0.05).Compared with the group B, the levels of NF-κB mRNA and serum VEGF decreased significantly in the groups C, D, E, and F, and the levels of IκB mRNA in the groups C, D, E and F increased significantly (P＜0.05).Conclusion Shengmai powder can significantly decrease the level of insulin resistance and VEGF in serum by inhibiting the serum NF-κB/IκB expression.关键词
2型糖尿病/生脉散/胰岛素抵抗/NF-κB/IκB/血管内皮生长因子Key words
type 2 diabetes mellitus/Shengmai powder/insulin resistance/nuclear factor-κB/inhibitor nuclear factor-κB/vascular endothelial growth factor分类
医药卫生引用本文复制引用
吴小慧,刘菲,段忠心..生脉散对2型糖尿病大鼠胰岛β细胞NF-κB/IκB及VEGF表达的影响[J].山东医药,2018,58(48):45-48,4.基金项目
湖南省中医药科研计划项目 (2013SZY03) （2013SZY03）
