山东医药2019，Vol.59Issue(2)：6-9,4.DOI:10.3969/j.issn.1002-266X.2019.02.002
还原型谷胱甘肽对辐射损伤小鼠外周血细胞及肠损伤的影响
Effects of reduced glutathione on peripheral blood cells and intestinal injury of irradiated mice
摘要
Abstract
Objective To observe the effects of different doses of reduced glutathione ( GSH) on peripheral blood cells and intestinal injury of irradiated mice. Methods C57BL/6 mice were randomly assigned into five groups: groups A1, B1, C1, D1 and E1. The mice in the group A1, group B1, and group C1 received intraperitoneal injection of 200, 100, and 50 mg/kg GSH 30 min before irradiation; mice in the group D1 were injected with normal saline intraperitoneally30 min before irradiation; and mice in the groups A1, B1, C1, and D1 received whole body irradiation of 4 Gy; and mice in the group E1 were injected with normal saline intraperitoneally as controls. Fifteen days after irradiation, the mice were sacrificed, hematology analyzer was used to measure cell counts in the peripheral blood, and flow cytometry was used to analyze the percentage of lymphocytes in the peripheral blood. C57BL/6 mice were randomly assigned into five groups: group A2, group B2, group C2, group D2, and group E2. The method of administration was the same as before. The mice in the group A2, group B2, group C2, and group D2 received irradiation of 10 Gy for the abdominal regions. Three days after irradiation, the mice were sacrificed, the small intestines of the mice were taken out, and the pathological changes of the small intestine were observed by HE staining. Results Compared with group E1, the number of WBCs, RBCs, PLTs and the percentage of T cells and B cells in the peripheral blood of the group D1 significantly decreased ( P < 0. 05 or P <0. 01) . Compared with group D1, the number of RBCs and the percentage of T cells and B cells in the peripheral blood of the group C1 increased ( all P < 0. 05) , and the number of WBCs, RBCs, PLTs and the percentage of T cells and B cells in the peripheral blood were the most highest in the group B1 ( P < 0. 05 or P < 0. 01) , but changes in the number of WBCs, RBCs, PLTs and the percentage of T cells and B cells in the group A1 had no statistically significant difference.Compared with group E2, the small intestinal villi of the mice in the group D2 got shortened and shrunk; compared with group D2, the small intestinal villi of the mice in the group C2 were longer, and the villi structure of small intestine in the group B2 was intact, but the villi structure of small intestine in the group A2 was neither intact nor regular. Conclusion GSH can promote hematopoietic recovery in irradiated mice and reduce the the harmful effects of ionizing radiation on small intestinal mucosa, with the optimal dosage of 100 mg/kg.关键词
还原型谷胱甘肽/辐射防护剂/外周血细胞/肠损伤/急性放射病Key words
reduced glutathione/radioprotector/peripheral blood cells/intestinal injury/acute radiation sickness分类
医药卫生引用本文复制引用
李雪娇,田红旗..还原型谷胱甘肽对辐射损伤小鼠外周血细胞及肠损伤的影响[J].山东医药,2019,59(2):6-9,4.基金项目
天津市新药创制科技重大专项(17ZXXYSY00090) （17ZXXYSY00090）
中国医学科学院医学与健康科技创新工程(2017-I2M-3-019). （2017-I2M-3-019）
