摘要
Abstract
Objective To observe the effects of total flavonoids of Oxytropis falcata ( FOF) on apoptosis and Toll-like receptor 4 ( TLR4) /MyD88/NF-κB pathway in the gastric cancer cells. Methods The proliferation of gastric cancer cell lines SGC7901, MKN-49 P, AGS, MKN45 and KATOIII was detected by MTT assay. The SGC7901 cells with the lowest IC50 were taken as the experimental cells. The concentration of FOF was 10-40 μg/m L and the duration of action was 48 h.The SGC7901 cells in the logarithmic phase were divided into 6 groups. The cells in the LPS group were treated with 2 μg/m L TLR4 activator LPS. The cells in the ER + LPS group were first treated with 100 nmol/L TLR4 inhibitor Eritoran for 2h and then were added with 2 μg/m L LPS. The cells in the LFOF, MFOF, and HFOF + LPS groups were first treated with10, 20, and 40 μg/m L FOF for 2 h, followed by 2 μg/m L LPS, and the cells in the blank group were added with the same amount of solvent. After 48-hour treatment, the apoptosis rate was measured by flow cytometry. The apoptosis-related proteins ( Bax, Bcl-2, Survivin) and TLR4/MyD88/NF-κB pathway-related proteins ( TLR4, p-TLR4, MyD88, p-MyD88, p65, and p-p65) were detected by Western blotting. The cell immunofluorescence was used to observe the nuclear importation of p65 protein. Results Compared with the blank group, the apoptosis rate of SGC7901 cells in the LPS group decreased, the expression of Bax protein decreased, the expression of Bcl-2, Survivin, p-TLR4, p-MyD88 and p-p65 protein increased, and the amount of p65 increased ( all P < 0. 05) . Compared with LPS group, the apoptosis rate of SGC7901 cells in the ER + LPS group, LFOF + LPS group, MFOF + LPS group, and HFOF + LPS group increased, Bax protein expression increased, the expression of Bcl-2, Survivin, p-TLR4, p-MyD88, and p-p65 proteins decreased, and the amount of p65 decreased ( all P < 0. 05) . Conclusion FOF can induce apoptosis of gastric cancer SGC7901 cells, which is related to the inhibition of TLR4/MyD88/NF-κB pathway activity.关键词
镰形棘豆总黄酮/胃癌细胞/TLR4/MyD88/NF-κB通路/细胞凋亡Key words
total flavonoids of Oxytropis falcate/gastric carcinoma cells/TLR4/MyD88/NF-κB pathway/apoptosis分类
医药卫生
