山东医药2019，Vol.59Issue(2)：32-35,4.DOI:10.3969/j.issn.1002-266X.2019.02.008
姜黄素对人肺鳞癌细胞NCI-H292黏蛋白5AC表达的影响及机制
Effect of curcumin on expression of mucin 5AC in human lung squamous cell carcinoma cell line NCI-H292 and its mechanism
摘要
Abstract
Objective To investigate the effect of curcumin on the expression of mucin 5AC ( MUC5AC) in human lung squamous cell carcinoma cell line NCI-H292 and its mechanism. Methods The cells were divided into two groups:the curcumin group and control group, and curcumin and DMSO were added, respectively. The expression levels of MUC5 AC, forkhead box protein A2 ( FOXA2) and phosphorylation signal transduction and activator protein 6 ( p-STAT6) were detected by Western blotting; the cells were divided into two groups: FOXA2 siRNA group and NC-siRNA group, which were transfected with FOXA2 siRNA and negative-siRNA for 24 h, respectively. The expression levels of MUC5 AC and FOXA2 were detected by Western blotting. The transfected two groups were treated with curcumin and the same amount of DMSO for 48 h, respectively, and we detected MUC5 AC by Western blotting. The cells were divided into the control group, inhibitor group and curcumin group, which were treated with DMSO, STAT6 inhibitor Kaempferol, and curcumin for48 h, respectively. The FOXA2 mRNA was detected by RT-PCR. Results Compared with the control group, the expression of MUC5 AC and p-STAT6 decreased and the expression of FOXA2 increased in the curcumin group ( all P < 0. 01) .Compared with the NC-siRNA group, FOXA2 expression level decreased and MUC5 AC protein expression increased in the FOXA2 siRNA group ( both P < 0. 01) . Compared with the cells treated with DMSO, the expression of MUC5 AC decreased after adding curcumin in the NC-siRNA group and FOXA2 siRNA group ( all P < 0. 01) . The relative expression of FOXA2 mRNA was as follows: control group < inhibitor group < curcumin group ( P < 0. 01) . Conclusion Curcumin up-regulates the FOXA2 protein expression by inhibiting STAT6 phosphorylation into the nucleus, thereby inhibiting the secretion of MUC5 AC by NCI-H292 cells.关键词
肺鳞癌/姜黄素/黏蛋白5AC/叉头框蛋白A2/信号转导及转录激活蛋白6Key words
lung squamous cell carcinoma/curcumin/mucin 5AC/forkhead box protein A2/signal transduction and activator protein 6分类
医药卫生引用本文复制引用
汤玲玲,倪振华,陈清阁,孟子誉,王雄彪..姜黄素对人肺鳞癌细胞NCI-H292黏蛋白5AC表达的影响及机制[J].山东医药,2019,59(2):32-35,4.基金项目
上海市科学技术委员会科研计划项目(18140904000) （18140904000）
上海市普陀区临床重点专科建设项目(2016PTZK03) （2016PTZK03）
上海市普陀区卫生系统自主创新科研资助项目(ptkwws201714) （ptkwws201714）
上海市普陀区中心医院专科医师规培科研课题资助项目(PTZP201614A). （PTZP201614A）
