山东医药2019，Vol.59Issue(4)：14-18,5.DOI:10.3969/j.issn.1002-266X.2019.04.004
SHC介导的胰岛素信号转导MAPK途径与先天性心脏病的关系
Correlation between SHC-mediated insulin signal transduction MAPK pathway and congenital heart disease
摘要
Abstract
Objective To observe the correlation between the abnormal insulin signal transduction pathway and the occurrence of no syndrome-congenital heart disease (ns-CHD). Methods The right atrium tissues from 5 cases of nsCHD patients (case group) and 2 cases of rheumatic heart disease (RHD) patients with mitral stenosis (MS) were detected by digital gene expression profiling (DGE), then we verified them in the right atrium tissues from 37 cases of ns-CHD patients and 12 cases of RHD MS patients by using real-time fluorescence quantitative PCR (RT-QPCR). Results SHC gene family-mediated MAPK pathway was restrained in the case group, and the expression of 6 genes (PTPRF, SHC4, ARAF, MAP2 K2, MKNK2, and ELK1) was significantly down-regulated (all P ＜ 0. 05). Conclusions Hence SHC-mediated MAPK pathway may influence the heart development. The relationship between SHC and insulin resistance may be one of the reasons for the high rate of neonatal CHD in gestational diabetes mellitus (GDM) neonates.关键词
先天性心脏病/妊娠期糖尿病/基因表达调控/SHC基因家族/MAPK途径Key words
congenital heart disease/gestational diabetes mellitus/gene expression regulation/SHC gene family/MAPK pathway分类
医药卫生引用本文复制引用
夏丽滨,石鑫,沈艳,陈艳,骆志玲..SHC介导的胰岛素信号转导MAPK途径与先天性心脏病的关系[J].山东医药,2019,59(4):14-18,5.基金项目
国家自然科学基金资助项目(81660052) （81660052）
云南省应用基础研究项目(2017FE468) （2017FE468）
