山东医药2019，Vol.59Issue(4)：19-22,4.DOI:10.3969/j.issn.1002-266X.2019.04.005
氢醌染毒对红白血病细胞株K562细胞周期的影响及其机制
Effect of hydroquinone on cell cycle of erythroleukemia cell line K562
摘要
Abstract
Objective To investigate the effect of hydroquinone (HQ) on the cell cycle of erythroleukemia cell line K562 and its mechanism. Methods K562 cells in the logarithmic phase were selected and then divided into the blank control group and HQ-treated low-dose group, medium-dose group and high-dose group which were treated with 0, 15, 30, and 60 μmol/L HQ for 72 h at intervals and repeatedly. After 72 h, the cell cycle distribution was detected by flow cytometry, and cell cycle-related protein expression such as CDC25 A, CDK2, CyclinE, CyclinA, p53, p21 and PCNA was detected Western blotting. Results The results of flow cytometry showed that the ratio of cells in the G0/G1 phase of the blank control group and low-dose, medium-dose and high-dose HQ groups was 55. 90％ ± 2. 71％, 40. 16％ ± 3. 34％, 29. 97％± 1. 23％, and 32. 84％ ± 0. 93 ％, and the ratio of cells in the S phase was 43. 42％ ± 2. 61％, 57. 01％ ± 3. 50％, 66. 87％ ± 1. 77％, and 66. 66％ ± 0. 31％, and the ratio of cells in the G2/M phase was 0. 73％ ± 0. 78％, 3. 27％ ±2. 05％, 4. 40％ ± 5. 07％, and 0. 18％ ± 1. 54％, respectively. Therefore, HQ could decrease the proportion of cells in the G0/G1 phase in the K562 cell cycle (P ＜ 0. 05), increase the proportion of cells of the S phase (P ＜ 0. 05), and induce S phase arrest. Western blotting showed that compared with the blank control group, the expression of CDC25 A protein decreased in each HQ dose group (P ＜ 0. 05), and the expression of the high-dose to medium-dose and low-dose HQgroups decreased (P ＜ 0. 05). Compared with the blank control group, the expression of CyclinE and PCNA protein in the low-dose group was not statistically different, but the expression in the medium-dose and high-dose HQ groups decreased (P ＜ 0. 05). The expression of CyclinE and PCNA protein from the high-dose group to medium-does group and low-dose groups decreased in turn after exposure of HQ (P ＜ 0. 05). Compared with the blank control group, the expression of p53 protein in the low-does and medium-dose groups increased (P ＜ 0. 05), and the difference in the high-dose group was not statistically significant. There was no significant difference in the expression of CDK2, CyclinA or P21 protein between the blank control group and HQ dose groups (all P＞ 0. 05). Conclusion HQ can induce S phase arrest in K562 cells which might be related to the down-regulated expression of CDC25 A, CyclinE, and PCNA.关键词
红白血病/苯/氢醌/S期阻滞/周期相关蛋白Key words
erythro leukemia/Benzene/hydroquinone/S phase arrest/cell cycle-related proteins分类
医药卫生引用本文复制引用
赵雪,温缘缘,肖芸,万秀方,李攀登,王永红,郑丹,谢婷婷,张亚莉..氢醌染毒对红白血病细胞株K562细胞周期的影响及其机制[J].山东医药,2019,59(4):19-22,4.基金项目
国家自然科学基金资助项目(81360437) （81360437）
