山东医药2019，Vol.59Issue(5)：1-4,4.DOI:10.3969/j.issn.1002-266X.2019.05.001
下调ANKRD22表达对人肺腺癌细胞增殖、凋亡及细胞周期的影响
Effects of down-regulating ANKRD22 expression on proliferation, apoptosis, and cell cycle of human lung adenocarcinoma cells
摘要
Abstract
Objective To investigate the effects of down-regulating the expression of ankyrin repeat domain 22 (ANKRD22) on the proliferation, apoptosis, and cell cycle of human non-small-cell lung cancer (NSCLC) H1975 cells.Methods H1975 cells were randomly divided into the the siRNA interference group, negative control group, and blank control group. We transfected the ANKRD22-siRNA and NC-siRNA into the siRNA interference group and negative control group by Lipofectamine 2000 reagent; the blank control group was not transfected. The expression level of ANKRD22 was detected by qRT-PCR. MTT assay was used to detect the proliferation of cells cultured for 24, 48, 72, 96 and 120 h. Flow cytometry was used to detect the apoptotic rate and cell cycle. Results The expression level of ANKRD22 in the siRNA interference group was significantly lower than that in the blank control group and negative control group (both P < 0.05) , and no significant difference was found between the blank control group and negative control growp, P> 0.05; the cell proliferation rate in the siRNA interference group was significantly lower than that in the blank control group and negative control group (P < 0.05); the proportion of cells in the G0/G1 phase of the siRNA interference group was significantly higher than that in the blank control group and negative control group, and the proportion of cells in S phase and G2/M phase was significantly lower than that in the blank control group and negative control group; the apoptosis rate of the siRNA interference group was significantly higher than that of the blank control group and the negative control group (P < 0.05); no sig-nificant differences were found in the above parameters between the blank control group and negative control group (all P>0.05). Conclusion The down-regulation of ANKRD22 expression can inhibit the proliferation of H1975 cells significantly, promote the apoptosis, and prolong the cell cycle.关键词
非小细胞肺癌/锚蛋白重复系列22/细胞增殖/细胞凋亡/细胞周期Key words
non-small-cell lung cancer/ankyrin repeat domain 22/cell proliferation/apoptosis/cell cycle分类
医药卫生引用本文复制引用
张烽,殷俊,傅文凡,戴璐,潘雷,钟圣鹏,赵健..下调ANKRD22表达对人肺腺癌细胞增殖、凋亡及细胞周期的影响[J].山东医药,2019,59(5):1-4,4.基金项目
国家自然科学基金项目资助项目(81572258) （81572258）
广州市科技计划项目(201607010031) （201607010031）
广州市属高校科研项目青年项目(1201630087) （1201630087）
