山东医药2019，Vol.59Issue(5)：29-32,4.DOI:10.3969/j.issn.1002-266X.2019.05.008
长链非编码RNA DANCR在结肠癌组织细胞中表达及其对LOVO细胞侵袭能力的影响
Expression of long non-coding RNA DANCR in colon cancer and its effect on invasion ability of LOVO cells
李涛 1王江 1王舒 1朱玄2
作者信息
- 1. 中国医科大学第七临床学院抚顺矿务局总医院,辽宁 抚顺 113008
- 2. 中国医科大学附属第一医院鞍山医院,辽宁 抚顺 113008
- 折叠
摘要
Abstract
Objective To observe expression of long non-coding RNA (lncRNA) differentiation antagonizing noncoding RNA (DANCR) in the colorectal cancer (CRC) cells and to explore its effect on the cell invasion. Methods The expression of DANCR in the CRC tissues and paired para-tumor tissues was determined by qRT-PCR. DANCR expression in the CRC patients with different clinicopathological features was measured. The qRT-PCR was applied to evaluate the expression of DANCR in the CRC cell line LOVO and normal epithelial cell line NCM460. LOVO cells were divided into the control group and DANCR-silenced group (or si-DANCR group) randomly, and were transfected with si-con and si-DANCR, respectively. The expression of DANCR was detected by qRT-PCR. We used the Transwell invasion assay to count the number of transmembrane cells and evaluate cell invasion. Western blotting was applied to detect the expression of invasion and metastasis-related factor matrix metalloproteinase 9 (MMP9). Results The expression of DANCR in the CRC tissues was higher than that in the para-tumor tissues. Additionally, the expression of DANCR in the LOVO cells was higher than that in the NCM460 cells (both P < 0.01). The expression of DANCR in the CRC patients with advanced clinical staging (stage Ⅲ and Ⅳ) , higher T staging (T3 and T4) , and distant metastasis was higher than that in CRC patients with early clinical staging (stage Ⅰ and Ⅱ) , lower T staging (T1 and T2) , and non-distant metastasis (P < 0.01). Compared with the control group, the expression of DANCR, the transmembrane cells, and the expression of MMP9 protein decreased in the si-DANCR group (all P < 0.01). Conclusion LncRNA DANCR is up-regulated in CRC tissues and cells, and DANCR works as an oncogene in the progression of CRC; DANCR promotes the metastatic ability of CRC cells, which is relatedto the up-regulation of MMP9.关键词
结肠癌/长链非编码RNA/分化拮抗非编码RNA/肿瘤侵袭Key words
colon carcinoma/long non-coding RNA/differentiation antagonizing non-coding RNA (DANCR)/tumor invasion分类
医药卫生引用本文复制引用
李涛,王江,王舒,朱玄..长链非编码RNA DANCR在结肠癌组织细胞中表达及其对LOVO细胞侵袭能力的影响[J].山东医药,2019,59(5):29-32,4.
