山东医药2019，Vol.59Issue(5)：37-39,43,4.DOI:10.3969/j.issn.1002-266X.2019.05.010
慢性阻塞性肺疾病大鼠肺组织中IGF-1、IGFBP3表达及其与气道重塑的关系
Expression of IGF-1 and IGFBP3 in lung tissues of rats with chronic obstructive pulmonary disease and its relationship with airway remodeling
唐诗帅 1张伟兵 1田晓彦 1陈晓明 1李佳宁 1何秋馥1
作者信息
- 1. 哈尔滨医科大学附属第四医院,哈尔滨 150001
- 折叠
摘要
Abstract
Objective To observe the expression of insulin-like growth factor 1 (IGF-1) and insulin-like growth factor binding protein 3 (IGFBP3) in the lung tissues of rats with chronic obstructive pulmonary disease (COPD) , and to explore the mechanism of airway remodeling. Methods Twenty male Wistar rats were randomly divided into model group and control group, with 10 in each. We adopted the method of cigarette smoking to establish the COPD rat models. The rats were put into the smoking box with 10 cigarettes in, each time we lit them for 30 minutes, 3 times/d, for 90 days. The control group was fed normally. The rats were killed and lung tissues were taken, the histomorphological changes were observed by HE staining and the wall thickness of small airway was measured. The expression of IGF-1 and IGFBP3 protein in each group were detected by immunohistochemistry. The expression of IGF-1 and IGFBP3 mRNA was detected by real-time fluorescence quantitative PCR; Pearson method was used to analyze the correlation between IGF-1 and IGFBP3 protein expression and bronchiole wall thickness. Results Compared with the control group, the bronchiole wall thickness in the model group increased, and the protein and mRNA expression levels of IGF-1 and IGFBP3 in the lung tissues were enhanced (all P < 0.01). IGF-1 protein and IGFBP3 protein were both positively correlated with the thickness of bronchiole wall (r =0.658 and 0.654, respectively; all P < 0.01). IGF-1 protein was positively correlated with IGFBP3 protein (r = 0.810, P< 0.01). Conclusion The expression of IGF-1 and IGFBP3 in the lung tissues of COPD rats increases, which may be involved in the airway remodeling of COPD by promoting the proliferation of cells around the airway.关键词
慢性阻塞性肺疾病/气道重塑/胰岛素样生长因子1/胰岛素样生长因子结合蛋白3Key words
chronic obstructive pulmonary disease/airway remodeling/insulin-like growth factor/insulin-like growth factor binding protein 3分类
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唐诗帅,张伟兵,田晓彦,陈晓明,李佳宁,何秋馥..慢性阻塞性肺疾病大鼠肺组织中IGF-1、IGFBP3表达及其与气道重塑的关系[J].山东医药,2019,59(5):37-39,43,4.
