山东医药2019，Vol.59Issue(5)：40-43,4.DOI:10.3969/j.issn.1002-266X.2019.05.011
黄连素对溃疡性结肠炎大鼠的治疗作用及其机制
Therapeutical effect of berberine on ulcerative colitis rats and its mechanism
摘要
Abstract
Objective To investigate the effect and mechanism of berberine in the treatment of ulcerative colitis (UC) in rats. Methods Twenty-four SD rats were divided into three groups (n = 8): the control group, model group, and berberine group. The rats in the model group and berberine group were given 5.5％ dextran sulfate sodium (DSS) solution to drink freely, forming the UC rat models. The rats in the control group were given distilled water to drink freely for seven days. In the next seven days, the control group and the model group were given distilled water by gavage, and the berberine group was given berberine (100 mg/kg). The degree of disease activity (DAI score) and the pathological changes of colon tissues in each group were observed. The serum IL-9 level was measured by ELISA, and the reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the expression levels of TLR2 mRNA and TLR2 protein in rat colon tissues. Results Compared with the control group, the DAI score, the colon histopathology score, the serum IL-9 level, TLR2 mRNA and TLR2 protein expression increased in the model group and berberine group (all P < 0.01). Compared with the model group, the DAI score on day 14 and the colon histopathology score decreased, and the serum IL-9 level, mRNA and protein expression of TLR2 decreased in the berberine group (all P < 0.05). Conclusions Berberine improves gastrointestinal symptoms and promotes intestinal mucosal healing in rat with UC. Berberine may play an important role in the treatment of UC by reducing the level of IL-9 and the mRNA and protein expression of TLR2.关键词
溃疡性结肠炎/黄连素/白细胞介素9/Toll样受体2/大鼠Key words
ulcerative colitis/berberine/interleukin-9/Toll-like receptor 2/rats分类
医药卫生引用本文复制引用
张君红,李洪梅,黄雪,井洁,张湘莲,余明华,石榴..黄连素对溃疡性结肠炎大鼠的治疗作用及其机制[J].山东医药,2019,59(5):40-43,4.基金项目
广西中医药民族医药自筹经费科研课题(GZZC15-29) （GZZC15-29）
