山西中医学院学报2018，Vol.19Issue(6)：21-24,4.
助阳开郁方对抑郁症大鼠海马BDNF表达及其胞内信号转导通路的影响
Effects of Zhuyang Kaiyu formula on BDNF expression and intracellular signal transduction pathway in rat hippocampus with depression
摘要
Abstract
Objective:To explore the mechanism of Zhuyang Kaiyu formula (ZKF) in the treatment of depression based on cyclic adenosine monophosphat (cAMP) -cAMP response-element binding protein (CREB) -bursa-derived neurotropic factor (BDNF) signal transduction pathway in hippocampus. Methods:36 male rats were randomly divided into normal group, model group, low-dose, middle-dose, high-dose ZKF groups and fluoxetine group, with 6 rats in each group. The rats in low-dose, middle-dose and high-dose ZKF groups were given aqueous solution of ZKF 11.7 g/kg, 23.4 g/kg, 46.8 g/kg respectively, the rats in fluoxetine group were given aqueous solution of 3.33 mg/kg fluoxetine hydrochloride dispersion tablets, while the rats in normal control group and model group were given solution. Except for normal control group, rats in all the other groups were received gavage administration and chronic unpredictable mild stress (CUMS) combined with isolation stimulus simultaneously for 28 days. The rats hippocampal tissues were taken out after drug intervention, the content of cAMP in depression rat hypothalamu tissue was detected by ELLSA, and the mRNA expressions of BDNF and CREB were detected by RT-PCR. Results:The contents of cAMP and the mRNA expressions of BDNF and CREB in the model group were significantly decreased compared to normal group. Compared with model group, the contents of cAMP in high-dose ZKF group and fluoxetine group were increased obviously, and expressions of CREB and BDNF mRNA in middle-dose and high-dose ZKF groups were significantly increased. Conclusion:The expression of signal molecules in hippocampal cAMP-CREB-BDNF transduction pathway was abnormal in depression rats, the antidepressant effects of ZKF may be correlated with its effect on the expression of BDNF and the expression and function of signal molecules in intracellular signal transduction pathway which is regulated in hippocampus.关键词
抑郁症/助阳开郁方/脑源性神经营养因子/环磷酸腺苷/环磷酸腺苷反应元件结合蛋白/海马Key words
depression/Zhuyang Kaiyu formula/bursa-derived neurotropic factor/cyclic adenosine monophosphate/cAMP response-element binding protein/hippocampal分类
医药卫生引用本文复制引用
冯振宇,赵杰,马小娟,王虹娟,陈佳丽..助阳开郁方对抑郁症大鼠海马BDNF表达及其胞内信号转导通路的影响[J].山西中医学院学报,2018,19(6):21-24,4.基金项目
山西省卫生和计划生育委员会科研课题 (2015093) （2015093）
