山西中医学院学报2018，Vol.19Issue(6)：27-30,35,5.
基于两种一测多评法对尾叶香茶菜中四种二萜类成分的质量控制研究
Quality control for four kinds of diterpenoids from Rabdosia excisa based on quantitative analysis of multi-components by single marker
摘要
Abstract
Objective:To establish a HPLC method to determine four active components (Kamebakaurin, excisanin A, rabdokunmin C and Kamebanin) in Rabdosia excisa (Maxim.). The methodological investigation mode of QAMS was established to verify the feasibility and technical adaptability of this approach in quality control of Rabdosia excisa. Methods:Four components were used as index components, two correction methods were used to establish the relative correction factor (fk/s) between each component and Kamebakaurin. Then fk/s was used to calculate the content of each component in Rabdosia excisa. Meanwhile external standard method was used to determine the content of the four compounds and the difference between calculated value and measured value was compared to verify the accuracy and feasibility of QAMS.Results:The methodological investigation mode of QAMS which was used to evaluate the four kinds of diterpenoids of Rabdosia excisa was established. There was not significant difference between the value calculated by multipoint correction with different instruments and columns and the values measured by external standard method. But there was significant difference between the value calculated by slope correction and the values measured by external standard method. Conclusion:Multipoint correction for calculating the content of Kamebakaurin, excisanin A, rabdokunmin C and Kamebanin in Rabdosia excisa is accurate and reliable, it could be used to control the quality of Rabdosia excise.关键词
一测多评/尾叶香茶菜/Kamebakaurin/excisanin A/rabdokunmin C/KamebaninKey words
quantitative analysis of multi-components by single marker (QAMS)/Rabdosia excisa (Maxim.) Hara/Kamebakaurin/excisanin A/rabdokunmin C/Kamebanin分类
医药卫生引用本文复制引用
杨丽霞,陈勇强,孙舰..基于两种一测多评法对尾叶香茶菜中四种二萜类成分的质量控制研究[J].山西中医学院学报,2018,19(6):27-30,35,5.基金项目
国家自然科学基金资助项目 (21501078) （21501078）
