山西中医学院学报2019，Vol.20Issue(1)：13-16,4.DOI:10.19763/j.cnki.1671-0258.2019.01.005
二至天癸颗粒对超排卵小鼠GDNF、GFR-1的影响
Effects of Er-zhi Tian-gui granule on expression of GDNF、GFR-l in Granulosa Cell of Superovulation Cycle
摘要
Abstract
Objective:In view of GVBD and extrusion of PB1, to explore the mechanism of Er-zhi Tiangui (ETG) granule to improve the quality of oocyte, the ratio of good quality embryo in superovulation cycle. Method:165 Kunming female mice were divided into three groups randomly. The mice in treatment group and control group were given HMG and HCG for ovarian stimulation, 0.9％ salt solution and ETG were respectively administered by gastric infusion to control group and treatment group on the same time schedule. The mice in blank group were given nothing. Three groups were divided into1 st group、2nd group each. All mice in 1st group were sacrificed and denuded oocytes (Dos) on germinal vesicle stage were taken out to observe extrusion of PB1. The oocytes were collected to detect expression of GDNF、GFR-1mRNA. The mice in 2nd group of treatment group and control group mated by rate of 1∶1 after the day of HCG injection, mice were retrieved to evaluate the ability of merogenesis and forming the embryo in the next day. The mice in blank group mated by rate of 1∶1on the day of estrus cycle, On the next day when vaginal suppository were found, then zygotes were retrieved to evaluate the ability of merogenesis and forming the embryo. Result:The ratio of germinal veicles breakdown and extrusion of the first polar body in treatment group was significantly higher than those of control group. The rate a of cleavage and forming the embryo in control group were lower than those of the treatment group (P＜0.05). The expression of GFRa-l mRNA in the oocytes of the treatment group was higher than that of the control group (P＜0.05). Conclusion:ETG can improve the embryo quality may be related to its effect on germinal veicles breakdown and extrusion of the first polar body, and can improve the expression of GFR-1 mRNA in oocytes.Then ETG can improve the ability of merogenesis and forming the embryo of fertilized ovum.关键词
二至天癸颗粒/超排卵/GDNF/GFR-1mRNAKey words
Erzhi Tian-gui (ETG) granule/superovulation cycle/GDNF/GFR-lmRNA分类
医药卫生引用本文复制引用
陈艳花,连方,张雪洛,柳俊珍,崔向荣..二至天癸颗粒对超排卵小鼠GDNF、GFR-1的影响[J].山西中医学院学报,2019,20(1):13-16,4.基金项目
山西省卫生健康委员会项目 (201301038) （201301038）
