上海针灸杂志2019，Vol.38Issue(2)：119-126,8.DOI:10.13460/j.issn.1005-0957.2019.02.0119
隔药灸对克罗恩病大鼠结肠NLRP3炎症小体及IL-1β调节作用的实验研究
Experimental Study on the Regulating Effect of Herbal Cake-partitioned Moxibustion on NLRP3 Inflammasome and IL-1β in Colons of Crohn's Disease Rats
摘要
Abstract
Objective To explore the action mechanism of moxibustion in treating Crohn's disease (CD) by observing the effect of herbal cake-partitioned moxibustion on the expression of NLRP3 inflammasome (NLRP3, ASC and Caspase-1) and IL-1β. Method Male Sprague Dawley (SD) rats of clean conventional grade were randomized into a normal control group (NG), a model control group (MG), a herbal cake-partitioned moxibustion control group (MoxG), and a sham moxibustion control group (SMG). The CD rat models were developed by using the mixture of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) and alcohol via enema. When the models were successfully developed, the MoxG received moxibustion intervention at bilateral Tianshu (ST25) and Qihai (CV6), and the SMG were administered by unlighted moxa cones. At the end of treatment, the length of each rats' colon and the colon macroscopic damage index (CMDI) score were recorded, the histopathological variations of rats' colons were observed by adopting HE staining and light microscope, and the expressions of NLRP3, ASC, Caspase-1 and IL-1β in rats' colons were determined by using immunohistochemical technique. Result Compared to the NG, the MG had its rats' colons present with severe damages, fissured ulcers and inflammatory cell infiltration with edema, and granulomas in submucosa of some colons, and its expressions of NLRP3, ASC, Caspase-1 and IL-1β increased significantly (P<0.05);compared to the MG, the MoxG had rats' colons present with improved structures and reduced intestinal inflammation, and its expressions of NLRP3, ASC, Caspase-1 and IL-1β dropped significantly (P<0.05); the SMG had its rats' colon inflammation present similarly to the MG, and its expressions of NLRP3, ASC, Caspase-1 and IL-1β had no significant difference (P>0.05). Conclusion Herbal Cake-partitioned moxibustion can down-regulate the expressions of NLRP3, ASC, caspase-1 and IL-1β in CD rats' colons to promote the repair of colon damage.关键词
药饼灸疗法/克罗恩病/NLRP3炎症小体/间接灸/大鼠Key words
Medicinal cake-partitioned moxibustion/Crohn's disease/NLRP3 inflammasome/Indirect moxibustion/Rats分类
医药卫生引用本文复制引用
张霁,马晓芃,吴丽洁,李志元,张丹,智方圆,杨延婷,吴丹艳,赵越,李茜莹..隔药灸对克罗恩病大鼠结肠NLRP3炎症小体及IL-1β调节作用的实验研究[J].上海针灸杂志,2019,38(2):119-126,8.基金项目
国家重点基础研究发展计划(2015CB554501) （2015CB554501）
国家自然科学基金项目(81674073) （81674073）
上海市卫生计生系统优秀学科带头人培养计划项目(2017BR047) （2017BR047）
