水生生物学报2019，Vol.43Issue(2)：259-263,5.DOI:10.7541/2019.032
禁食与胰岛素处理对鳜血糖和leptin-A基因表达的影响(英文)
EFFECTS OF FASTING AND INSULIN TREATMENT ON BLOOD GLUCOSE AND LIEPTIN-A GENE EXPRESSION IN CHINESE PERCH
摘要
Abstract
Leptin has been reported to play important roles in regulation of glucose homeostasis in mammals. However, the relationship between blood glucose and leptin gene expression in teleosts is largely unknown. In the present study, blood glucose and leptin-A gene expression in liver, intestine and visceral adipose tissue were characterized in Chinese perch (Siniperca chuatsi) after fasting or insulin treatment. In the fasting experiment, Chinese perch were fasted for 10 days, sampling at 0, 4h, 2d, 6d and 10d after fasting. Blood glucose was significantly decreased at 10d after fasting; leptin-A mRNA expression was significantly increased in liver at 6d after fasting, and significantly increased in intestine and visceral adipose tissue at 4h after fasting. In the insulin treatment experiment, Chinese perch were intraperitoneally injected with bovine insulin (80 IU/kg body weight) or 0.7％ saline as control, sampling at 12h and 36h post treatment. Blood glucose decreased significantly at 12h after insulin treatment, and leptin-A mRNA expression in intestine increased significantly at 36h after insulin treatment, which suggesting that prolonged fasting and insulin treatment decreased blood glucose, and increased leptin-A gene expression in fat depot organ and digestive organ of fish.关键词
瘦素/血糖/禁食/胰岛素/鳜Key words
Leptin-A/Blood glucose/Fasting/Insulin/Siniperca chuatsi分类
生物科学引用本文复制引用
李玲,梁旭方,何珊,张焱鹏,陈旭,沈丹..禁食与胰岛素处理对鳜血糖和leptin-A基因表达的影响(英文)[J].水生生物学报,2019,43(2):259-263,5.基金项目
China Agriculture Research System(CARS-46) （CARS-46）
