水生生物学报2019，Vol.43Issue(2)：264-271,8.DOI:10.7541/2019.033
三角鲂×翘嘴鲌、团头鲂×翘嘴鲌两种杂交后代微卫星遗传结构分析
MICROSATELLITE MARKERS ANALYSIS OF TWO HYBIRDS OF MEGALOBRAMA TERMINALIS ×ERYTHROCULTER ILISHAEFORMIS, MEGALOBRAMA AMBLYCEPHALA× ERYTHROCULTER ILISHAEFORMIS
摘要
Abstract
To guide breeding of Megalobrama terminalis, Megalobrama amblycephala and Erythroculter ilishaeformis, two hybrids Megalobrama amblycephala×Erythroculter ilishaeformis, Megalobrama terminalis×Erythroculter ilishaeformis and their parents were analyzed using sixteen microsatellite markers. The mean value of number of alleles (Na) were 3.56, 3.63, 3.44, 4.00 and 4.31, respectively. The mean value of observed heterozygosity (Ho) were 0.3510, 0.3757, 0.3175, 0.3818, and 0.4079, respectively. The mean value of expected heterozygosity (He) were 0.6182, 0.6290, 0.5921, 0.6490 and 0.6825, respectively. And the average value of polymorphism information content (PIC) were 0.5354, 0.5367, 0.5258, 0.5785, and 0.6067, respectively. The average value of polymorphism information content (PIC) of two hybrid population were higher than their parent Megalobrama amblycephala, Megalobrama terminalis and Erythroculter ilishaeformis, indicating a high genetic diversity. The unweighted pair-group method with arithmetic means (UPGMA) showed that populations of the Megalobrama amblycephala and Megalobrama terminalis first grouped together, and that Megalobrama amblycephala♀×Erythroculter ilishaeformis♂ and Megalobrama terminalis♀×Erythroculter ilishaeformis♂ first grouped together, and then they clustered with the Erythroculter ilishaeformis. In addition, the farthest genetic distance (0.5204) was between Megalobrama amblycephala and Erythroculter ilishaeformis, and the nearest genetic distance (0.0853) was between Megalobrama amblycephala and Megalobrama terminalis. According to the genetic identity analysis, two hybirds groups showed the maternal effect. The alleles of two hybirds were from their parents based on genetic analysis. Particularly, microsatellite primers TTF3, TTF4, TTF10, and Mam25 identified 5 groups. Our results provide important value in breeding, germplasm resources preservation, and germplasm identification of Megalobrama terminalis×Erythroculter ilishaeformis and Megalobrama amblycephala×Erythroculter ilishaeformis.关键词
团头鲂/三角鲂/翘嘴鲌/遗传结构/微卫星Key words
Megalobrama amblycephala/Megalobrama terminalis/Erythroculter ilishaeformis/Genetic structure/Microsatellite分类
生物科学引用本文复制引用
苏晓磊,郑国栋,蒋霞云,邹曙明..三角鲂×翘嘴鲌、团头鲂×翘嘴鲌两种杂交后代微卫星遗传结构分析[J].水生生物学报,2019,43(2):264-271,8.基金项目
国家自然科学基金(31272633、31201760、31572220) （31272633、31201760、31572220）
上海高校知识服务平台(ZF1206)资助 （ZF1206）
