水生生物学报2019，Vol.43Issue(2)：298-304,7.DOI:10.7541/2019.037
三疣梭子蟹NF-κB家族基因Relish和Dorsal的克隆及表达特征
CLONING AND EXPRESSION OF NF-κB FAMILY GENES IN PORTUNUS TRITUBERCULATUS
摘要
Abstract
Relish and Dorsal, members of nuclear factor-κB (NF-κB) family protein, play crucial role in immune response in Arthropoda. In this study, the cDNA sequences of Relish and Dorsal were cloned by rapid amplification of cDNA ends (RACE). There are 3254 nucleotides in Pt-Rel with a putative open reading frame (ORF) of 2949 bp that encodes a polypeptide of 983 amino acid residues. The Pt-Dor cDNA was 2348 bp in length including an ORF encoding a polypeptide of 637 amino acid residues.Prediction of protein domains revealed that both Pt-Rel and Pt-Dor contained typical NF-κB family structures RHD (Rel homology domain) and IPT (Immunoglobulin-like fold, Plexins, Transcription factors). Alignment of amino acid sequences for Pt-Rel and Pt-Dor revealed high identities with other arthropodas orthologs. Phylogenetic tree analysis based on amino acid sequences showed that the Pt-Rel and Pt-Dor were distinctly clustered with crustaceans Relish and Dorsal, respectively, but separated from insect orthologs. Pt-Rel and Pt-Dor were expressed in all tested tissues with the highest level in hemocytes. Three different pathogenic microorganism significantly induced both Pt-Rel and Pt-Dor expression in primary cultured crab hemocytes at 2h by Candida lusitaniae infection and 4h by Staphylococcus aureus and Vibrio alginolyticus challenge. These data suggest that Pt-Rel and Pt-Dor might be involved in host defense against to microbial pathogens in Portunus trituberculatus.关键词
三疣梭子蟹/NF-κB/基因克隆/表达分析Key words
Portunus trituberculatus/NF-κB/Clone/Expression分类
生物科学引用本文复制引用
赵姣姣,陶震,周素明,金珊,王国良,周岐存..三疣梭子蟹NF-κB家族基因Relish和Dorsal的克隆及表达特征[J].水生生物学报,2019,43(2):298-304,7.基金项目
宁波市农业重大项目(2017C110007) （2017C110007）
现代农业产业技术体系专项资金(CARS-48)资助 （CARS-48）
