水生态学杂志2018，Vol.39Issue(5)：67-75,9.DOI:10.15928/j.1674-3075.2018.05.010
雅砻江硬刺松潘裸鲤的胚胎及仔鱼发育研究
Embryonic and Larval Development of Gymnocypris potanini firmispinatus in Yalong River
摘要
Abstract
Gymnocypris potanini firmispinatus is an indigenous cold-water fish inhabiting the Jinsha River.The development of cascaded hydropower stations, overfishing and environment pollution have resulted in sharp declines in wild populations of G.p.firmispinatus.In this paper, we describe and illustrate the embryonic/larval development of G.p.firmispinatus and compare its morphology and early-stage growth characteristics with other Schizothoracinae species.The objective was to provide theoretical evidence for large-scale artificial reproduction and seedling cultivation.Parental stock was collected from Yalong River from July, 2015 to February, 2016, with the following characteristics:age (female, 6-13 yr;male 3-9 yr), total length (female, 12.1-24.2 cm;male, 8.2-14.5 cm), body weight (female, 16.1-115.8 g;male, 5.2-23.8 g).The zygotes were obtained by dry fertilization, and the incubation of zygotes and the culture of yolk-sac larvae were conducted under controlled conditions:temperature, (16±1) ℃;dissolved oxygen,>7.0 mg/L.At least 30 zygotes and 10 larvae were sampled during the development period and the development sequence and primary morphological characteristics of each stage were recorded.Results show that fertilized eggs were ovate and yellow, with an average diameter of (2.82±0.10) mm.The entire embryonic development period was 183 h45 min at (16±1) ℃and the maximum cumulative temperature was 2 889 h℃.The development process consisted of eight stages:egg fertilization, blastoderm formation, cleavage, blastula, gastrula, neurula, organ development and release of newly hatched larvae from the membrane.The average total length of newly hatched larvae was (7.8±0.19) mm.Approximately 10 days after hatching, the gill, mouth, pectoral fin, tail fin, swim bladder, intestinal tract and other organs gradually developed.Yolk-sac absorption was complete after day 13, when the average total length of the larvae reached (14.33±0.5) mm.Forty days after hatching, all fins were formed and body shape and color were similar to adults, indicating that the G.p.firmispinatus hatchlings had developed into juveniles.G.p.firmispinatus is primarily distributed in the stem and tributaries of the middle Yalong River and exhibits a series of morphological and ecological adaptations to its habitat.In this study, we found that the early development of G.p.firmispinatus also presents certain adaptations to the habitat.关键词
硬刺松潘裸鲤/胚胎发育/仔鱼发育/雅砻江Key words
Gymnocypris potanini firmispinatus/embryonic development/larval development/Yalong River分类
生物科学引用本文复制引用
徐滨,魏开金,朱祥云,甘维熊,邓龙君,徐进,马宝珊,郭峰..雅砻江硬刺松潘裸鲤的胚胎及仔鱼发育研究[J].水生态学杂志,2018,39(5):67-75,9.基金项目
中国水产科学研究院基本科研业务费资助项目(2016JBF0303) （2016JBF0303）
雅砻江流域水电开发有限公司科研项目(JPIAG201225). （JPIAG201225）
