生物加工过程2019，Vol.17Issue(2)：131-137,7.DOI:10.3969/j.issn.1672-3678.2019.02.003
盐单胞菌S62 β-半乳糖苷酶合成低聚半乳糖的研究
Synthesis of galactooligosaccharides by β-galactosidase from Halomonas sp. S62
摘要
Abstract
We used the Halomonas sp. S62 β-galactosidase to synthesize galactooligosaccharides from lactose, to explore galactooligosaccharides synthesis efficiency. To improve the yield of galactooligosaccharides, the reaction conditions were optimized, and the orthogonal test was carried out by the reaction temperature, p H, enzyme dosage and substrate concentration. The optimal reaction conditions were as follows: reaction temperature 40 ℃, p H 7. 0, enzyme dosage 50 U/m L, substrate concentration 300 g/L.The maximal yield of galactooligosaccharides was (41. 91±0. 27) ％ at 6 h and the lactose consumption rate was (82. 47±0. 38) ％.Within 4-8 h, the yield of galactooligosaccharides was above 40％ and the lactose consumption rate was over80％.The yield of galactooligosaccharides was maintained above 40％ within 4-8 h of the reaction. The consumption rate of lactose was all above 80％. The utilization rate of lactose was increased while the high production of galactooligosaccharides was achieved, which was beneficial to the reduction of production cost, laying the foundation for the industrial application of low-temperature S62 β-galactosidase.关键词
β-半乳糖苷酶/低聚半乳糖产率/乳糖消耗率/盐单胞菌Key words
β-galactosidase/galactooligosaccharides yield/lactose consumption rate/Halomonas sp.分类
轻工纺织引用本文复制引用
朱五二,缪明永,顾正华,李由然,丁重阳,石贵阳..盐单胞菌S62 β-半乳糖苷酶合成低聚半乳糖的研究[J].生物加工过程,2019,17(2):131-137,7.基金项目
国家高技术研究发展计划(863计划)(2014AA093513) （863计划）
江苏省科技项目(BN2016202) （BN2016202）
