生物加工过程2019，Vol.17Issue(2)：138-143,6.DOI:10.3969/j.issn.1672-3678.2019.02.004
响应面法优化链霉菌S10A09发酵产纤维素酶条件
Optimization of Streptomyces sp. S10A09 for cellulase production by response surface method
摘要
Abstract
Strains of actinomycetes were screened for their carboxymethyl cellulase (CMCase) productivity.Streptomyces sp. S10 A09 was chosen to be the best producing strain.In order to obtain higher filter paper activity, Plackett-Burman and Center Composite Design of response surface methodology were used to optimize conditions of cellulase production by Streptomyces sp.S10 A09.CMC-Na and (NH4) 2SO4 were the most significant factors improving enzyme production.The Center Composite Design results showed that the optimum medium was as follows:CMC-Na 2.57 g/L, (NH4) 2SO4 11.31 g/L, KH2PO4 0.2 g/L, MgSO4 1 g/L, FeSO4 0.01 g/L.Under the above conditions, the filter paper activity was 125.96 U/mL, 3 times as that before the optimization.关键词
纤维素酶/Plackett-Burman/响应面法/滤纸酶活/链霉菌Key words
cellulase/Plackett-Burman/center composite design/response surface method/filter paper activity/Streptomyces sp分类
生物科学引用本文复制引用
霍光明,张李阳,朱枳穆,俞丽娜..响应面法优化链霉菌S10A09发酵产纤维素酶条件[J].生物加工过程,2019,17(2):138-143,6.基金项目
江苏省农业三新工程项目(SXGC[2016]022) （SXGC[2016]022）
江苏省高校重点建设实验室项目(苏教科[2016]8) （苏教科[2016]8）
